The antimicrobial resistance crisis requires novel approaches for the therapy of infections especially with Gram-negative pathogens. Pseudomonas aeruginosa is defined as priority 1 pathogen by the WHO and thus of...

In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in highthroughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.