In this paper we present the ZooCAM, a system designed to digitize and analyse on board large volume samples of preserved and living metazooplankton (i.e. multicellular zooplankton) and fish eggs > 300 µm ESD. The ZooCAM has been specifically designed to overcome the difficulties to analyse zooplankton and fish eggs in the framework of the PELGAS survey, and provide high frequency data. The ZooCAM fish eggs counts were comparable to those done with a dissecting microscope. The ZooCAM enabled the accurate prediction and fast on board validation of staged anchovy and sardine eggs in almost real time after collection. A comparison with the ZooScan, on a more complex zooplanktonic community, provided encouraging results on the agreement between the 2 instruments. ZooCAM and ZooScan enabled the identification of similar communities and produced similar total zooplankton abundances, size distributions, and size spectra slopes, when tested on the same samples. However these results need to be further refined due to the small number of samples used to compare the two instruments. The main ZooCAM drawback resides in a slight but sensible underestimation of abundances and sizes, and therefore individual and community biovolumes. The ZooCAM have been successfully deployed over 4 years, on numerous surveys without suffering any major failure. When used in line with the CUFES it provided high resolution maps of staged fish eggs and zooplanktonic functional groups. Hence the ZooCAM is an appropriate tool for the development of on board, high frequency, high spatial coverage zooplanktonic and ecosystemic studies. Highlights ► The ZooCAM is a new inflow imaging system for fast onboard counting, sizing and classification of fish eggs and metazooplankton. ► The ZooCAM has been used on-board for 4 years without suffering any failure and enabled the analysis ∼10,000 samples so far (CUFES, WP2 and other plankton nets). ► The ZooCAM provided staged fish eggs counts that were equivalent to those traditionally done by microscopic examination. These data were used for 2016 and 2017 anchovy and sardine stocks estimations in the Bay of Biscay (DEPM method) ► The ZooCAM enable the real time, on-board, imaging of complex communities zooplankton samples and provide results comparable to on-land, well established counterpart, the ZooScan. ► The ZooCAM is a valuable tool to improve zooplanktonic studies, in term of spatial coverage and temporal frequency, to perform ecosystemic studies.

in the Sea of Marmara to gain detailed insight into the fate of fluids migrating within the sedimentary column and partially released into the water column. The overall objective of the project was to achieve a more global understanding of cold-seep dynamics in the context of a major active strike-slip fault. Five remotely operated vehicle (ROV) dives were performed at selected areas along the North Anatolian Fault and inherited faults. To efficiently detect, select and sample the gas seeps, we applied an original procedure. It combines sequentially (1) the acquisition of ship-borne multibeam acoustic data from the water column prior to each dive to detect gas emission sites and to design the tracks of the ROV dives, (2) in situ and real-time Raman spectroscopy analysis of the gas stream, and (3) onboard determination of molecular and isotopic compositions of the

Un drone nautique a été développé pour la surveillance des eaux côtières et estuariennes dans le cadre de la Directive Cadre sur l'eau (DCe) pour :-aller du rivage à un point de prélèvement situé à 500 m en moins de 5 minutes,-effectuer des mesures in situ,-prélever des échantillons d'eau pour une analyse ultérieure au laboratoire (identification des espèces phytoplanctoniques, chlorophylle, nutriments,…). Mots-clés : drone nautique, prélèvement d'eau, eaux côtières et estuariennes Development of a nautical drone for coastal and estuarine water sampling ABSTRACT.-A nautical drone was designed for the monitoring of estuarine and coastal waters in the context of the Water Framework Directive (WFD) to :-go up to 500 m off the coastline in less than 5 minutes,-perform in situ measurements (temperature, salinity, turbidity),-collect water samples for later analysis in the laboratory (phytoplankton identification, chlorophyll, nutrients, ...).