Citizens' preferences form the foundation of democratic governance. When they form their preferences, they typically do so in the presence of various types of competing arguments that reach them at different times. Surprisingly, public opinion research offers little guidance on how competition and time affect preference formation. We fill this gap by exploring the relative influence of two prominent types of competing arguments, frames and cues, over time. We find that only frames have initial direct effects, although cues exert initial indirect effects on opinion formation. Over time, the relative impact of frames and cues depends on individual differences in processing style. Our results have important implications for opinion formation, political communication, and democratic responsiveness. Framing and Cue EffectsOur first task is to distinguish framing effects and cue effects. Typically, a framing effect occurs when in the course of describing a campaign, issue, problem, or event, a speaker's emphasis on a subset of potentially relevant considerations causes individuals to focus on

Background Identifying which taxa are targeted by immunoglobulins can uncover important host-microbe interactions. Immunoglobulin binding of commensal taxa can be assayed by sorting bound bacteria from samples and using amplicon sequencing to determine their taxonomy, a technique most widely applied to study Immunoglobulin A (IgA-Seq). Previous experiments have scored taxon binding in IgA-Seq datasets by comparing abundances in the IgA bound and unbound sorted fractions. However, as these are relative abundances, such scores are influenced by the levels of the other taxa present and represent an abstract combination of these effects. Diversity in the practical approaches of prior studies also warrants benchmarking of the individual stages involved. Here, we provide a detailed description of the design strategy for an optimised IgA-Seq protocol. Combined with a novel scoring method for IgA-Seq datasets that accounts for the aforementioned effects, this platform enables accurate identification and quantification of commensal gut microbiota targeted by host immunoglobulins. Results Using germ-free and Rag1−/− mice as negative controls, and a strain-specific IgA antibody as a positive control, we determine optimal reagents and fluorescence-activated cell sorting (FACS) parameters for IgA-Seq. Using simulated IgA-Seq data, we show that existing IgA-Seq scoring methods are influenced by pre-sort relative abundances. This has consequences for the interpretation of case-control studies where there are inherent differences in microbiota composition between groups. We show that these effects can be addressed using a novel scoring approach based on posterior probabilities. Finally, we demonstrate the utility of both the IgA-Seq protocol and probability-based scores by examining both novel and published data from in vivo disease models. Conclusions We provide a detailed IgA-Seq protocol to accurately isolate IgA-bound taxa from intestinal samples. Using simulated and experimental data, we demonstrate novel probability-based scores that adjust for the compositional nature of relative abundance data to accurately quantify taxon-level IgA binding. All scoring approaches are made available in the IgAScores R package. These methods should improve the generation and interpretation of IgA-Seq datasets and could be applied to study other immunoglobulins and sample types.

Rethinking Existentialism argues that the core of existentialism is the theory that Simone de Beauvoir and Jean-Paul Sartre described when they popularized the term in 1945: the ethical theory that we ought to treat human freedom as intrinsically valuable and the foundation of all other value. The book argues that Beauvoir and Sartre disagreed over the structure of this freedom in 1943 but that Sartre came to accept Beauvoir’s view by 1952, that Frantz Fanon’s first book should also be classified as a canonical work of existentialism, and that Beauvoir’s argument for a moral imperative of authenticity is a firmer ground for existentialism’s ethical claim than any of the eudaimonist arguments offered by Fanon and Sartre. It develops its arguments through critical contrasts with Albert Camus, Sigmund Freud, and Maurice Merleau-Ponty. The book concludes by sketching contributions that this analysis of existentialism can make to contemporary philosophy, psychology, and psychotherapy.

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