Juvenile salmon life history strategies, survival, and habitat interactions may vary by age cohort. However, aging individual juvenile fish using scale reading is time consuming and can be error prone. Fork length data are routinely measured while sampling juvenile salmonids. We explore the performance of aging juvenile fish based solely on fork length data, using finite Gaussian mixture models to describe multimodal size distributions and estimate optimal agediscriminating length thresholds. Fork length-based ages are compared against a validation set of juvenile coho salmon, Oncorynchus kisutch, aged by scales. Results for juvenile coho salmon indicate greater than 95% accuracy can be achieved by aging fish using length thresholds estimated from mixture models. Highest accuracy is achieved when aged fish are compared to length thresholds generated from samples from the same drainage, time of year, and habitat type (lentic versus lotic), although relatively high aging accuracy can still be achieved when thresholds are extrapolated to fish from populations in different years or drainages. Fork lengthbased aging thresholds are applicable for taxa for which multiple age cohorts coexist sympatrically. Where applicable, the method of aging individual fish is relatively quick to implement and can avoid ager interpretation bias common in scale-based aging.

Context Efficient restoration of longitudinal river connectivity relies on barrier mitigation prioritization tools that incorporate stream network spatial structure to maximize ecological benefits given limited resources. Typically, ecological benefits of barrier mitigation are 5 measured using proxies such as the amount of accessible riverine habitat.Objectives We developed an optimization approach for barrier mitigation planning which directly incorporates the ecology of managed taxa, and applied it to an urbanizing salmonbearing watershed in Alaska.Methods A novel river connectivity metric that exploits information on the distribution and 10 movement of managed taxon was embedded into a barrier prioritization framework to identify optimal mitigation actions given limited restoration budgets. The value of ecological information on managed taxa was estimated by comparing costs to achieve restoration targets across alternative barrier prioritization approaches.Results Barrier mitigation solutions informed by life history information outperformed those 15 using only river connectivity proxies, demonstrating high value of ecological information for watershed restoration. In our study area, information on salmon ecology was typically valued at 0.8-1.2M USD in costs savings to achieve a given benefit level relative to solutions derived only from stream network information, equating to 16-28% of the restoration budget.Conclusions Investing in ecological studies may achieve win-win outcomes of improved 20 understanding of aquatic ecology and greater watershed restoration efficiency.

Juvenile Pacific salmon exhibit diverse habitat use and migration strategies to navigate high environmental variability and predation risk during freshwater residency. Increasingly, urbanization and climate‐driven hydrological alterations are affecting the availability and quality of aquatic habitats in salmon catchments. Thus, conservation of freshwater habitat integrity has emerged as an important challenge in supporting salmon life‐history diversity as a buffer against continuing ecosystem changes. To inform catchment management for salmon, information on the distribution and movement dynamics of juvenile fish throughout the annual seasonal cycle is needed. A number of studies have assessed the ecology of juvenile coho salmon (Oncorhynchus kisutch) during summer and autumn seasons; catchment use by this species throughout the annual cycle is less well characterized, particularly in high‐latitude systems. Here, n = 3,792 tagged juvenile coho salmon were tracked throughout two complete annual cycles to assess basin‐wide distribution and movement behaviour of this species in a subarctic, ice‐bearing catchment. Juvenile coho salmon in the Big Lake basin, Alaska, exhibited multiple habitat use and movement strategies across seasons; however, summer rearing in lotic mainstem environments followed by migration to lentic overwinter habitats was identified as a prominent behaviour, with two‐thirds of tracked fish migrating en masse to concentrate in a small subset of upper catchment lakes for the winter. In contrast, the most significant tributary overwintering site (8% of tracked fish) occurred below a culvert and dam, blocking juvenile fish passage to a headwater lake, indicating that these fish may have been restricted from reaching preferred lentic overwinter habitats. These findings emphasize the importance of maintaining aquatic connectivity to lentic habitats as a conservation priority for coho salmon during freshwater residency.

In this study, we examined summer and fall freshwater rearing habitat use by juvenile coho salmon (Oncorhynchus kisutch) in the quickly urbanising Big Lake drainage in south-central Alaska. Habitat use was assessed by regressing fish count data against habitat survey information across thirty study sites using generalised linear mixed models. Habitat associations were examined by age-0 and age-1+ cohorts separately, providing an opportunity to compare habitat use across different juvenile coho salmon life stages during freshwater rearing. Regression results indicated that the age-0 cohorts were strongly associated with shallow, wide stream reaches with in-stream vegetation, whereas age-1+ cohorts were associated with deeper stream reaches.Furthermore, associations between fork length and habitat characteristics suggest cohort-specific habitat use patterns are distinct from those attributable to fish size.Habitat use information generated from this study is being used to guide optimal fish passage restoration planning in the Big Lake drainage. Evidence for habitat use partitioning by age cohort during freshwater juvenile rearing indicates that pooling age cohorts into a single "juvenile" stage for the purposes of watershed management may mask important habitat use dynamics. K E Y W O R D Scoho salmon, habitat use, cohort variation, generalised linear mixed model

Widespread stream network fragmentation from dams and culverts has altered habitat connectivity in river ecosystems and presents an acute threat to migratory fish. To support watershed management for an iconic migratory fish group, we assessed juvenile salmon growth outcomes across habitat use strategies and characterized how these life histories may be impacted by stream connectivity loss. Juvenile coho salmon (Oncorhynchus kisutch) in the Big Lake drainage, Alaska, USA, were individually tracked over 2012-2013 and categorized into habitat use behaviors, with fish either remaining in streams throughout freshwater residency or migrating seasonally to overwinter in lake habitats. Size, growth rate, and body condition of smolts (n = 1113) were compared across habitat use strategies. Juvenile coho salmon that moved seasonally to lake overwintering habitats, the most frequently observed strategy, grew faster and were significantly larger as smolts compared to their counterparts who remained in streams exclusively (spring Age 1 fish: 18% larger by weight, 9% faster growth rate; spring Age 2+ fish: 26% heavier, 11% faster