BackgroundWhile next-generation sequencing (NGS) costs have fallen in recent years, the cost and complexity of computation remain substantial obstacles to the use of NGS in bio-medical care and genomic research. The rapidly increasing amounts of data available from the new high-throughput methods have made data processing infeasible without automated pipelines. The integration of data and analytic resources into workflow systems provides a solution to the problem by simplifying the task of data analysis.ResultsTo address this challenge, we developed a cloud-based workflow management system, Closha, to provide fast and cost-effective analysis of massive genomic data. We implemented complex workflows making optimal use of high-performance computing clusters. Closha allows users to create multi-step analyses using drag and drop functionality and to modify the parameters of pipeline tools. Users can also import the Galaxy pipelines into Closha. Closha is a hybrid system that enables users to use both analysis programs providing traditional tools and MapReduce-based big data analysis programs simultaneously in a single pipeline. Thus, the execution of analytics algorithms can be parallelized, speeding up the whole process. We also developed a high-speed data transmission solution, KoDS, to transmit a large amount of data at a fast rate. KoDS has a file transfer speed of up to 10 times that of normal FTP and HTTP. The computer hardware for Closha is 660 CPU cores and 800 TB of disk storage, enabling 500 jobs to run at the same time.ConclusionsClosha is a scalable, cost-effective, and publicly available web service for large-scale genomic data analysis. Closha supports the reliable and highly scalable execution of sequencing analysis workflows in a fully automated manner. Closha provides a user-friendly interface to all genomic scientists to try to derive accurate results from NGS platform data. The Closha cloud server is freely available for use from http://closha.kobic.re.kr/.

서 론서울의 AbstractPlatform screen doors (PSD) installed at 289 stations in Seoul metropolitan subway from November 2005 to December 2009, are expected to prevent death from a fall and improve air quality. In this study, we systematically surveyed changes in radon concentrations before and after PSD installation in Seoul metropolitan subway stations. By solid-state nuclear track detectors (SSNTD), the radon concentrations before and after the PSD installation were measured at 54 stations of 6 lines from 2 to 7 reported to have relatively high radon concentrations. Mean radon concentrations at platforms were decreased by approximately 56% from 121.7 Bq/m 3 to 54.0 Bq/m 3 . Before PSD installation, mean radon concentrations were in the decreasing order for subway lines 7, 5, 6, 3, 4 and 2. On the other hand, after PSD installation the order was changed to 5, 6, 7, 3, 4 and 2. According to a radon map of Seoul metropolitan subway, the number of platforms where radon concentration over was 74 Bq/m 3 decreased from 38 to 12 after PSD installation.

Functional analyses of genes are crucial for unveiling biological responses, for genetic engineering, and for developing new medicines. However, functional analyses have largely been restricted to model organisms, representing a major hurdle for functional studies and industrial applications. To resolve this, comparative genome analyses can be used to provide clues to gene functions as well as their evolutionary history. To this end, we present Prometheus (http://prometheus.kobic.re.kr),web-based omics portal that contains more than 17,215 sequences from prokaryotic and eukaryotic genomes. This portal supports interkingdom comparative analyses via a domain architecture-based gene identification system, Gene Search, and users can easily and rapidly identify single or entire gene sets in specific pathways. Bioinformatics tools for further analyses are provided in Prometheus or through BioExpress, a cloud-based bioinformatics analysis platform. Prometheus suggests a new paradigm for comparative analyses with large amounts of genomic information.

IM(Instant Messenger) chatting service can carry user's various information including life style, geographical position, and psychology & crime history and thus forensic analysis on the IM service is desirable. But, forensic analysis for KakaoTalk's chatting service is not well studied yet. For this reason, we study KakaoTalk's forensic analysis focusing on chatting service. This paper first details a general method of IM forensics investigating the previous articles about IM forensics although there are not many articles. Second, we discuss methodologies for IM forensics wherein we present analysis of table structure and method for reconstruction of chatting message. These result in the basic element of forensic tools of KakaoTalk chatting message. Last, we compare artifacts of KakaoTalk with that of WhatsApp. We conclude that these applications are, at least, different in that table structures and the ways to reconstruct chatting messages are not same and therefore digital evidences or artifacts are not same and somewhat distinct.

The installation of the expansion joints in a tunnel concrete lining and duct would minimize the cracking at the location of structural shape and stiffness change, differential settlement, big temperature change, and so on. However, it is difficult to determine the required spacing of the expansion joint in a tunnel concrete lining and duct quantitatively because the spacing is influenced by temperature change, structure construction condition, ground-structure interaction, and etc.Nevertheless, a highway specification (Korea Expressway Corporation, 2012) or a road design manual (Ministry of Land, Transport and Maritime Affairs, 2010) specifies that the expansion joint spacing in a tunnel concrete lining should be installed uniformly smaller than 25 m from the tunnel portals to 50 m inside of a tunnel and elsewhre 20-60 m in a tunnel (because there is no specifcation for a duct it is assumed that a duct follows the specfication of lining). This specification results in several construction and economic problems in relation with a tunnel construction. Accordingly, in order to minimize the problems, this study analyzed both domestic and foreign design standards and specifications.In addition, field test, theoretical and numerical analyses were carried out in relation to the expansion joint in a tunnel lining and duct. The purpose of this study is to reestabilish a criterion for installing the expansion joint in a tunnel concrete lining and duct.