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The performances of tetracycline based cation selective polymeric membrane electrodes of many sets with different plasticizers were investigated as the selectivity of ion-selective electrodes and optodes are greatly influenced by membrane solvent and also controlled by plasticizers. The membrane 1 with Bis(2-ethylhexyl) sebacate (DOS) and additive shows good potentiometric performance toward Ca 2+ (slope: 27.8 mV per decade; DL: −4.52) including selectivity. Contrastingly, membrane 4 with Dibutyl phthalate (DBP) shows nearNernstian response, it has also shown the best measuring range and detection limit for Ca 2+ (29.5 mV and −5.10) and Mg 2+ (24.4 mV and −5.04) and the least selectivity has been also observed between Ca 2+ and Mg 2+. When the membrane 1 and 4 were used together to flow system, we could determine the concentration of Ca 2+ and Mg 2+, simultaneously.

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