No abstract

Chinese hamster ovary (CHO) cells are the most prevalent mammalian cell factories for producing recombinant therapeutic proteins due to their ability to synthesize human-like post-translational modifications and ease of maintenance in suspension cultures. Currently, a wide variety of CHO host cell lines has been developed; substantial differences exist in their phenotypes even when transfected with the same target vector. However, relatively less is known about the influence of their inherited genetic heterogeneity on phenotypic traits and production potential from the bioprocessing point of view. Herein, we present a global transcriptome and proteome profiling of three commonly used parental cell lines (CHO-K1, CHO-DXB11, and CHO-DG44) in suspension cultures and further report their growth-related characteristics, and N-and O-glycosylation patterns of host cell proteins (HCPs). The comparative multi-omics and subsequent genome-scale metabolic network model-based enrichment analyses indicated that some physiological variations of CHO cells grown in the same media are possibly originated from the genetic deficits, particularly in the cell-cycle progression. Moreover, the dihydrofolate reductase deficient DG44 and DXB11 possess relatively less active metabolism when compared to K1 cells. The protein processing abilities and the N-and O-glycosylation profiles also differ significantly across the host cell lines, suggesting the need to select host cells in a rational manner for the cell line development on the basis of recombinant protein being produced. K E Y W O R D S CHO parental cell lines, critical quality attributes, mammalian systems biotechnology, multi-omics analysis, N-glycosylation

In action recognition research, two primary types of information are appearance and motion information that is learned from RGB images through visual sensors. However, depending on the action characteristics, contextual information, such as the existence of specific objects or globally-shared information in the image, becomes vital information to define the action. For example, the existence of the ball is vital information distinguishing “kicking” from “running”. Furthermore, some actions share typical global abstract poses, which can be used as a key to classify actions. Based on these observations, we propose the multi-stream network model, which incorporates spatial, temporal, and contextual cues in the image for action recognition. We experimented on the proposed method using C3D or inflated 3D ConvNet (I3D) as a backbone network, regarding two different action recognition datasets. As a result, we observed overall improvement in accuracy, demonstrating the effectiveness of our proposed method.