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This study was conducted to understand the relationship between familiarity and cross-cultural acceptance for an ethnic sweet treat (Yackwa; Korean traditional cookie) by Korean, Japanese and French consumers. Descriptive analysis and consumer testing were performed on six Yackwa samples. Overall, the samples received favorable responses from the foreign consumers. Korean consumers liked samples with a soft and cohesive texture, whereas Japanese and French consumers liked flaky and crispy texture. French consumers rated stronger sweetness to be more appropriate for Yackwa compared to Korean and Japanese consumers. Texture liking was strongly correlated with familiarity rating in all three countries, indicating that the consumers' previous experience with similar products might affect their preference for certain textural attributes. Familiarity was correlated with all hedonic ratings by Korean consumers, who are most familiar with Yackwa, but with overall and texture liking by Japanese consumers and flavor and texture liking by French consumers. These results suggest that familiarity partly contributes to a foreign consumers' hedonic rating. PRACTICAL APPLICATIONSGlobalization and cultural diversity have increased interest in ethnic foods. This trend is motivating food industries to expand into the ethnic food market sector. In this study, the sensory attributes and the cross-cultural acceptability of Yackwa (Korean traditional cookie) were evaluated and the potential role of familiarity in determining consumer acceptance was measured. The outcome of this study will help food exporters, R&D scientists and food marketers in ethnic food market to optimize an ethnic food for other cultural communities by educating them to consider familiarity as an important factor for product development and promotion. bs_bs_banner Journal of Sensory Studies * Standard deviation values are shown in parentheses. The highest and the lowest mean scores for a given sensory attribute were highlighted in bold. † Means of four replicates from the eight panelists; mean values within a row not sharing a superscript letter are significantly different (P < 0.05, Duncan's multiple range test). CROSS-CULTURAL ACCEPTANCE OF YACKWA J.H. HONG ET AL.

BackgroundSeveral recent studies showed that next-generation sequencing (NGS)-based human leukocyte antigen (HLA) typing is a feasible and promising technique for variant calling of highly polymorphic regions. To date, however, no method with sufficient read depth has completely solved the allele phasing issue. In this study, we developed a new method (HLAscan) for HLA genotyping using NGS data.ResultsHLAscan performs alignment of reads to HLA sequences from the international ImMunoGeneTics project/human leukocyte antigen (IMGT/HLA) database. The distribution of aligned reads was used to calculate a score function to determine correctly phased alleles by progressively removing false-positive alleles. Comparative HLA typing tests using public datasets from the 1000 Genomes Project and the International HapMap Project demonstrated that HLAscan could perform HLA typing more accurately than previously reported NGS-based methods such as HLAreporter and PHLAT. In addition, the results of HLA-A, −B, and -DRB1 typing by HLAscan using data generated by NextGen were identical to those obtained using a Sanger sequencing–based method. We also applied HLAscan to a family dataset with various coverage depths generated on the Illumina HiSeq X-TEN platform. HLAscan identified allele types of HLA-A, −B, −C, −DQB1, and -DRB1 with 100% accuracy for sequences at ≥ 90× depth, and the overall accuracy was 96.9%.ConclusionsHLAscan, an alignment-based program that takes read distribution into account to determine true allele types, outperformed previously developed HLA typing tools. Therefore, HLAscan can be reliably applied for determination of HLA type across the whole-genome, exome, and target sequences.Electronic supplementary materialThe online version of this article (doi:10.1186/s12859-017-1671-3) contains supplementary material, which is available to authorized users.

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A practical synthesis of 2'-deoxy-2'-fluoro-5-methyl-beta-L-arabinofuranosyl uracil (14, L-FMAU) was developed from L-arabinose. L-Arabinose was converted to L-ribose 5, which was used for the synthesis of bromosugar 12 via 2,3,5-O-tribenzoyl-1-O-acetyl-beta-L-ribofuranose 8, which was subjected to condensation with silylated thymine and the resulting protected L-FMAU 13 was deprotected to afford L-FMAU in 14 steps in 8% overall yield.