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This study investigated the gelatinization process of polymeric suspensions of cassava starch (Manihot esculenta Crantz) plasticized with glycerol or ethylene glycol and used for biofilm production. Scanning electron microscopy confirmed that the starch, used as raw material for suspensions, consists of granule-forming clods and granular aggregates. Physical parameters such as viscosity, density, and temperature can be evaluated and used to accurately characterize and identify the gelatinization point of the polyol-plasticized starch. Upon reaching the gelatinization point, the suspensions went underwent retrogradation and had a kinetic viscosity of 19 to 23.508 mPa•s for the starch-glycerol suspension and 13.56 to 16.12 mPa•s for the starch-ethylene glycol suspension. However, the density of the suspensions slightly decreased during this process, ranging from 1.01 to 0.98 g/cm3. The starch-glycerol biofilm was more malleable and resistant, while the starch-ethylene glycol biofilm was inflexible and brittle. The use of different polyols facilitated the modification of the solubilization capacity of the biofilms. The starch-glycerol biofilm had a solubility value three times higher than that of the starch-ethylene glycol biofilm.

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