Aromatized wines and regular table wines are often filled on the same bottling line. Sealing polymers in the filling line absorb volatiles from aromatized wines and may migrate due to insufficient cleaning into the subsequently bottled regular wine. Unintentional carryover of volatiles may lead to accusation of illegal aromatization of wine. Absorption, cleaning efficacy, and migration of volatiles into ethylene propylene diene monomer rubber were investigated in a model system. Direct thermal desorption–gas chromatography–mass spectrometry analysis of seven aroma compounds monitored variation in the polymer (μg/g). Absorption of volatiles was mostly driven by their octanol/water partition coefficients. Cleaning of polymers removed 11 to 62% of the absorbed volatiles. Subsequent immersion of cleaned polymers into model wine revealed migration of 20 to 57% of the remaining volatiles. Sensory tests suggested the impact of transferred volatiles into subsequent model wine. For α-ionone, an odor activity value of 1.03 indicated a potential sensory impact.

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Background and objectives Various cereals, fruits, and vegetables are commonly contaminated with mycotoxins such as zearalenone (ZEN), alternariol (AOH), and alternariol monomethyl ether (AME). More recently, their glucosidic metabolites formed in the plant have gained increasing attention. Experimental data on the contribution of modified mycotoxins to total toxicity are either controversy or lacking. Thus, the aim of this study was to investigate the absorption and metabolism of ZEN‐, AOH‐, and AME‐glucosides using the Caco‐2 cell system. Findings No quantifiable amounts of ZEN‐14‐glucoside, ZEN‐16‐glucoside, free ZEN, and ZEN metabolites were found in Caco‐2 cells and in the basolateral compartment. In contrast, glucosides of AOH and AME were absorbed and released their parental toxins, which were further metabolized to form glucuronides and sulfates to a variable extent. Metabolites were found on the basolateral site, too. There is also evidence that AOH‐9‐diglucoside is hydrolyzed to AOH‐9‐glucoside. Conclusion Our results demonstrate that modified ZEN is less absorbed whereas modified Alternaria toxins are taken up to a higher extent by Caco‐2 cells, followed by deglucosylation, metabolization, and transport to the basolateral site, suggesting a potential contribution to overall toxicity of these modified mycotoxins. Significance and novelty For the first time, absorption studies using modified Alternaria toxins in the Caco‐2 cell system were carried out.