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ABSTRACT. Reproductive aspects of Dendropsophus sanborni (Anura, Hylidae) in northeastern Argentina. The aim of this investigation was to characterize reproductive aspects of Dendropsophus sanborni (Schmidt, 1944) (Anura, Hylidae) in the northwest of Corrientes province, in northeastern Argentina. The reproductive cycles of males and females, the existence of sexual dimorphism and the male's vocalization period were analyzed. Samplings were made from September 2011 to August 2012. Dendropsophus sanborni showed a prolonged reproductive pattern because reproductive activity was observed during most part of the year. Calling males were recorded all months except in July. The spermatogenic cycle can be characterized as potentially continuous. In males, snout-vent length was significantly correlated with mean testicular volume. The species showed sexual dimorphism in body mass, with females larger than males. Post-ovulatory females were found from March to June and most females had oocytes at different stages of maturity, with abundant post-vitellogenic oocytes from August to December. KEYWORDS.Reproduction, vocalizations, gonads. RESUMEN. Aspectos reproductivos deDendropsophus sanborni (Anura, Hylidae) en el nordeste de la Argentina. El objetivo del presente estudio fue caracterizar aspectos reproductivos de Dendropsophus sanborni (Schmidt, 1944) (Anura, Hylidae) en la región noroeste de la provincia de Corrientes, al Nordeste de Argentina. Para esto, se analizó el ciclo gonadal de machos y hembras, la existencia de dimorfismo sexual y el periodo de vocalización de los machos. Los muestreos se efectuaron desde Septiembre de 2011 hasta Agosto de 2012. Dendropsophus sanborni exhibe un patrón reproductivo prolongado, dado que la actividad reproductiva ocurre durante gran parte del año. Se registraron machos en actividad de vocalización durante todos los meses excepto en julio. El ciclo espermatogénico puede ser caracterizado como potencialmente continuo. En los machos hubo correlación significativa entre la longitud hocico-cloaca y el volumen testicular medio. La especie presentó dimorfismo sexual en relación a la masa corporal, siendo las hembras mayores que los machos. Se encontraron hembras post-ovulatorias desde Marzo a Junio y una mayor proporción de hembras con ovocitos en diferentes estados de maduración, con abundantes ovocitos post-vitelogénicos, en los meses de Agosto a Diciembre.

Oriental Chaco, part of the Great American Chaco, embraces a plain area with soft slopes towards the west-east region.

To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson's trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.

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