Guava is a fruit appreciated worldwide for its high content of bioactive compounds. However, it is considered a highly perishable fruit, generally attacked by pathogenic species such as the fungi Colletotrichum gloeosporioides, which causes anthracnosis. To diminish the losses caused by pathogenic fungi, coatings of chitosan (CS) with Ruta graveolens essential oil (RGEO) in different concentrations (0.5, 1.0, 1.5% v/v) were applied in situ and their effects on the physical properties and microbiological quality of the guavas were studied. The CS+RGEO coated fruits exhibited better physicochemical behavior and lower microbiological decay as compared to the uncoated guavas, demonstrating the effectiveness of the coatings, especially those with 1.5% of RGEO content. All the fruits coated had greater acceptance and quality than the controls, being more those with essential oil incorporation. In situ investigation of C. gloesporioides infection of guavas demonstrated that the CS+RGEO coated guavas showed a high percentage of inhibition in the development of anthracnose lesions. In the present investigation, an alternative method has been proposed to extend the stability of the guavas fruit up to 12 days with application in the food industry.

Recently, tissue engineering became a very important medical alternative in patients who need to regenerate damaged or lost tissues through the use of scaffolds that support cell adhesion and proliferation. Carbon nanomaterials (carbon nanotubes, fullerenes, multi-wall fullerenes, and graphene) became a very important alternative to reinforce the mechanical, thermal, and antimicrobial properties of several biopolymers. In this work, five different formulations of chitosan/poly(vinyl alcohol)/oxidized carbon nano-onions (CS/PVA/ox-CNO) were used to prepare biodegradable scaffolds with potential biomedical applications. Film characterization consisted of Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), tension strength, Young’s modulus, X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS). The degradation in a simulated body fluid (FBS) demonstrated that all the formulations lost between 75% and 80% of their weight after 15 days of treatment, but the degradation decreased with the ox-CNO content. In vivo tests after 90 days of subdermal implantation of the nanocomposite films in Wistar rats’ tissue demonstrated good biocompatibility without allergenic reactions or pus formation. There was a good correlation between FBS hydrolytic degradation and degradation in vivo for all the samples, since the ox-CNO content increased the stability of the material. All these results indicate the potential of the CS/PVA/ox-CNO nanocomposite films in tissue engineering, especially for long-term applications.

The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world. Tissue engineering offers multiple options from biocompatible materials with easy resorption. Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications. In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions. An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films. Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO. In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation. Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v). The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials. On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation. However, mechanical properties demonstrated an understandable loss of tensile strength and Young’s modulus for F3 and F4. However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films. The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed. All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.

Tissue engineering is crucial, since its early adoption focused on designing biocompatible materials that stimulate cell adhesion and proliferation. In this sense, scaffolds made of biocompatible and resistant materials became the researchers’ focus on biomedical applications. Humans have used essential oils for a long time to take advantage of their antifungal, insecticide, antibacterial, and antioxidant properties. However, the literature demonstrating the use of essential oils for stimulating biocompatibility in new scaffold designs is scarce. For that reason, this work describes the synthesis of four different film composites of chitosan/polyvinyl alcohol/tea tree (Melaleuca alternifolia), essential oil (CS/PVA/TTEO), and the subdermal implantations after 90 days in Wistar rats. According to the Young modulus, DSC, TGA, mechanical studies, and thermal studies, there was a reinforcement effect with the addition of TTEO. Morphology and energy-dispersive (EDX) analysis after the immersion in simulated body fluid (SBF) exhibited a light layer of calcium chloride and sodium chloride generated on the material’s surface, which is generally related to a bioactive material. Finally, the biocompatibility of the films was comparable with porcine collagen, showing better signs of resorption as the amount of TTEO was increased. These results indicate the potential application of the films in long-term biomedical needs.

The search for new biocompatible materials that can replace invasive materials in biomedical applications has increased due to the great demand derived from accidents and diseases such as cancer in various tissues. In this sense, four formulations based on polycaprolactone (PCL) and polylactic acid (PLA) incorporated with zinc oxide nanoparticles (ZnO-NPs) and tea tree essential oil (TTEO) were prepared. The sol-gel method was used for zinc oxide nanoparticle synthesis with an average size of 11 ± 2 nm and spherical morphology. On the other hand, Fourier Transformed infrared spectroscopy (FTIR) showed characteristic functional groups for each composite component. The TTEO incorporation in the formulations was related to the increased intensity of the C-O-C band. The thermal properties of the materials show that the degradative properties of the ZnO-NPs decrease the thermal stability. The morphological study by scanning electron microscopy (SEM) showed that the presence of TTEO and ZnO-NPs act synergistically, obtaining smooth surfaces, whereas membranes with the presence of ZnO-NPs or TTEO only show porous morphologies. Histological implantation of the membranes showed biocompatibility and biodegradability after 60 days of implantation. This degradation occurs through the fragmentation of the larger particles with the presence of connective tissue constituted by type III collagen fibers, blood vessels, and inflammatory cells, where the process of resorption of the implanted material continues.