Amostras de fezes de 280 cães e 30 gatos, de ambos os sexos, idades e raças variadas, foram examinadas pelo Laboratório de Análises Clínicas Veterinárias “Veterinária Preventiva” – Curitiba, PR, no período de 01 de Janeiro a 31 de Dezembro de 2005. As fezes foram colhidas individualmente e processadas pelos métodos Gordon; Whitlock (1939), Willis (1927) e Hoffman; Pons; Janer (1934). Das amostras de cães, 54 (19,28%) foram positivas com ocorrência de Isospora sp. (38,9%), Ancylostoma sp. (31,45%), Toxocara spp. (9,25%), Giardia sp. (11,1%), Trichuris sp. (5,60%), em infecções únicas. Observou-se infecções associadas de Isospora sp. e Dipylidium caninum (1,85%) e Taenia sp. e Giardia sp. (1,85%). Das amostras de gatos, apenas 4 foram positivas (13,33%) e os parasitas encontrados foram Isospora sp. (75%) e Toxocara spp. (25%). O parasita mais comum, nas amostras fecais dos cães e gatos, foi o Isospora sp..

Enzootic bovine leukosis (EBL) is a retroviral infection that causes persistent lymphocytosis and lymphosarcoma in cattle. The economic importance of infection by bovine leukemia virus (BLV) is due to several factors, including losses in exportation, treatment of secondary infection, and reduction in dairy production. To facilitate the development of a national test that is sensitive, simple, and applicable on a large scale, this work aimed to produce and characterize monoclonal antibodies (mAbs) against gp51 protein from BLV for use in an enzyme-linked immunosorbent assay (ELISA) test. Two hundred seventy-four hybridomas were generated, from which 37 were mAbs secretory clones screened by indirect ELISA. The specificity of the mAbs generated against gp51 was verified by Western blot analysis, and the isotypes were characterized for isotyping in IgG1 and IgM. To evaluate the test, 250 sera were tested by agar gel immunodiffusion and mAb-ELISA. The values obtained for the mAb-ELISA test were 95% sensitivity and 90% specificity.

The aim of this work was to produce and characterize monoclonal antibodies anti bovine immunoglobulin G (IgG

The objective of this study was to formulate an anti-rabies oral vaccine from the cell culture vaccine PV TECPAR to elicit the production of antibodies against the rabies in mice. A vaccine was developed using 10 7.5 DL 50 /0.03 ml viral antigens homogenised in lanovaseline to facilitate oral administration. Mice were vaccinated two times for seroconversion. Sera of the vaccinated mice showed a higher level of antibody production than the control group. These results could be used to direct the development of an anti-rabies oral vaccine.