The increasing cultivation of microalgae in photobioreactors warrants efficient and non-invasive methods to quantify biomass density in real time. Nephelometric turbidity assessment, a method that measures light scatter by particles in suspension, was introduced already several decades ago but was only recently validated as a high-throughput tool to monitor microalgae biomass. The light scatter depends on the density of the suspended particles as well as on their physical properties, but so far there are hardly any accounts on how nephelometric assessment relates to classic methods such as dry weight and spectrophotometric measurement across a broad biomass density range for different microalgae species. Here, we monitored biomass density online and in real time during the semi-continuous cultivation of three commercial microalgae species Chloromonas typhlos, Microchloropsis gaditana and Porphyridium purpureum in pilot-scale photobioreactors, and relate nephelometric turbidity to dry weight and optical density. The results confirm a relatively strong (R² = 0.87–0.93) and nonlinear relationship between turbidity and biomass density that differs among the three species. Overall, we demonstrate how nephelometry can be used to monitor microalgal biomass in photobioreactors, and provide the necessary means to estimate the biomass density of the studied species from turbidity data to facilitate automated biomass monitoring.

The most studied and cultivated microalgae have a temperature optimum between 20 and 35°C. This temperature range hampers sustainable microalgae growth in countries with colder periods. To overcome this problem, psychrotolerant microalgae, such as the snow alga Chloromonas typhlos, can be cultivated during these colder periods. However, most of the research work has been carried out in the laboratory. The step between laboratory-scale and large-scale cultivation is difficult, making pilot-scale tests crucial to gather more information. Here, we presented a successful pilot-scale growth test of C. typhlos. Seven batch mode growth periods were compared during two longer growth tests in a photobioreactor of 350 L. We demonstrated the potential of this alga to be cultivated at colder ambient temperatures. The tests were performed during winter and springtime to compare ambient temperature and sunlight influences. The growth and CO2 usage were continuously monitored to calculate the productivity and CO2 fixation efficiency. A maximum dry weight of 1.082 g L−1 was achieved while a maximum growth rate and maximum daily volumetric and areal productivities of 0.105 d−1, 0.110 g L−1 d−1, and 2.746 g m−2 d−1, respectively, were measured. Future tests to optimize the cultivation of C. typhlos and production of astaxanthin, for example, will be crucial to explore the potential of biomass production of C. typhlos on a commercial scale.