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This study aimed to evaluate the antinociceptive effect of sulphated polysaccharide from the marine algae Hypnea pseudomusciformis (PLS) using rodent models of orofacial pain. Acute pain was induced by formalin, capsaicin, cinnamaldehyde, acidified saline or glutamate (cutaneous modes) and hypertonic saline (corneal model). In one experiment, animals were pretreated with ruthenium red, glibenclamide, naloxone, L-NAME, methylene blue or ketamine to investigate the mechanism of antinociception. In another experiment, animals pretreated with PLS or saline were submitted to the temporomandibular joint formalin test. In yet another, animals were submitted to craniofacial pain induced by mustard oil. Motor activity was evaluated with the open-field test. Cytotoxicity and antioxidant activities were also assessed. Pre-treatment with PLS significantly reduced nociceptive behavior associated with acute pain. Antinociception was effectively reduced, but not inhibited, by ruthenium red and ketamine. L-NAME and glibenclamide enhanced the PLS effect. PLS antinociception was resistant to methylene blue, naloxone and heating. PLS presented no cytotoxicity or antioxidant properties. Our results confirm the potential pharmacological relevance of PLS as an inhibitor of orofacial nociception in acute pain probably mediated by glutamatergic, nitrergic, TRPs and K + ATP pathways.

O processo inflamatório participa de todos os estágios do desenvolvimento da lesão acnéica. As plantas medicinais produzem compostos bioativos que lhes conferem ações terapêuticas, podendo ser utilizadas no tratamento de afecções da pele. Este trabalho teve como objetivo elaborar e testar a eficácia antiinflamatória não clínica de uma formulação (gel-creme) contendo extratos glicólicos de Aloe vera, Ginkgo biloba L., Panax ginseng, Matricaria recutita e mel de abelha. Foram realizados estudos preliminares de estabilidade. As amostras foram analisadas com respeito às propriedades organolépticas, pH, centrifugação e gelo-degelo, durante 8 semanas. Foi realizada a avaliação do efeito anti-inflamatório tópico da formulação em modelo experimental de inflamação cutânea utilizando camundongos Swiss e C57BL⁄6J. Os animais receberam aplicações tópicas de óleo de Croton durante 9 dias e, a partir do 5º dia, foram tratados com o gel-creme teste ou gel-creme base (controle), duas vezes ao dia. A formulação apresentou boa estabilidade físico-química e inibiu o edema de orelha, tendo seu efeito anti-inflamatório mais evidente nos camundongos C57BL⁄6J. Este resultado sugere que a formulação é eficaz como agente anti-inflamatório tópico no processo inflamatório crônico, o que justificaria o seu uso no tratamento da acne.Palavras-chave: Gel-creme, Compostos biativos, Óleo de Croton.The inflammatory process appears to participate in all stages of the development of acne lesion. Medicinal plants produce bioactive compounds that impart numerous therapeutic actions, and they can be used for the treatment of skin diseases. This work aimed to develop and to test the non-clinical anti-inflammatory effect of a formulation (cream-gel) containing glycolic extracts of Aloe Vera, Ginkgo biloba, Panax ginseng, Matricaria recutita and honey bee. Preliminary stability studies were carried out. The samples were analyzed with respect to organoleptic properties, pH variation, centrifugation and ice-melt, for 8 weeks. The anti-inflammatory effect of the cream-gel was evaluated using experimental model of cutaneous inflammation in Swiss and C57BL⁄6J mice. Animals received topical application of Croton oil for 9 days and, from day 5, they were treated with the cream-gel test or cream-gel base (control), twice daily. The formulation presented good physical and chemical stability and it inhibited the ear edema and the response was more evident in the C57BL⁄6J strain. This result suggests that the formulation is effective as topical anti-inflammatory agent in chronic inflammatory process, justifying its use in the treatment of acne.Keywords: Cream-gel, Bioactive compounds, Croton oil. ____________________________________________________________________________________________________________ INTRODUÇÃOAcne vulgaris, conhecida como acne, é uma doença inflamatória crônica da unidade pilossebácea [1]. Características da acne incluem seborreia (excesso de produção de óleo), lesões não-inflamatórias (comedões abertos e fechados), lesões inflamatórias (pápul...