This study provides evidence of the complexity inherent in the use of micro-organisms in agriculture, while also confirming that the activity of the biofertilizers based on Trichoderma depends on the type of inoculum and its concentration, as well as the properties of the medium in which the fungi develop. Further studies assessing the effectiveness or possible pathogenicity of Trichoderma in different soils under greenhouse conditions must be addressed.

Asparagus Decline Syndrome (ADS) is one of the main phytosanitary problems of asparagus crop worldwide. Diseased plants and soil samples from 41 fields from three main production areas of Spain were surveyed. Eight Fusarium species belonging to seven species complexes were identified in soils: F. oxysporum, F. proliferatum, F. redolens, F. solanisensu stricto, F. equiseti, F. culmorum, F. compactum and F. acuminatum. Fusarium oxysporum was the most prevalent species. Statistical correlation (R2 = 88%) was established between F. oxysporum inoculum density and the average temperature of the warmest month. A relationship was also established between three crop factors (average temperature, crop age and F. oxysporum inoculum density) and field disease indices. Significant differences were observed between the distribution of F. oxysporum propagules in white and green asparagus fields. Thirteen Fusarium species belonging to seven species complexes were identified from roots of diseased plants, being F. oxysporum the most prevalent. F. proliferatum, F. oxysporum and F. redolens showed pathogenicity to asparagus and were the main species associated to ADS. Fusarium oxysporum was the species with the highest genetic diversity displaying 14 sequence-based haplotypes with no geographic differentiation. This work contributes to understanding the Fusarium complex associated to ADS for developing accurate integrated disease management strategies.

Intensive horticultural practices in the greenhouse can cause proliferation of soil phytopathogenic organisms and pollution of groundwater from nitrate leaching. Among the different soil disinfection techniques, bio-disinfection through the addition of organic amendments (OA), with subsequent solarisation (biosolarisation) or without (biofumigation), is an efficient and economically viable alternative for the control of soil pathogens. This greenhouse experiment was conducted to initiate the process of conversion of a conventional tomato culture to organic farming. The effects of OA applied through biofumigation and biosolarisation in the first season on soil nitrate concentration and tomato fruit yield and quality were evaluated with the following treatments: control, no OA; T1, 0.3 kg m–2 of dehydrated pellets of Brassica carinata seed meal; T2, 0.8 kg m–2 of packaged and dehydrated B. oleracea var. italica; T3, T2 + 0.15 kg m–2 of dehydrated poultry manure; T4, T1 + 0.16 L m–2 of microbial cocktail. The experiment was carried out over the autumn crop cycle of two seasons (2011–12, 2012–13). Addition of OA increased soil nitrate concentration, more so with biosolarisation. Total and marketable yield of tomato and number of marketable fruits were higher in the biosolarised plots in the first crop after treatments. No effects were observed in the second crop after treatments. Except for tomato fruit firmness, quality attributes (size, soluble solids, acidity and colour) improved with some OA treatments. The supply of OA through biosolarisation is a soil disinfection technique with potential to minimise the impact of nitrate leaching and to provide improved yield and quality of tomatoes.

Sustainability transitions in agriculture are explored through an analysis of niche initiatives within a common production system, relying on sustainable transitions, multi-level perspectives, and agroecological frameworks, and involving multi-actor, agricultural knowledge, and innovation systems (AKIS). The article focuses on how experimental niches and sustainable activities affect farmers’ relationships with nature, and the reconceptualisation of the production system in which they operate, particularly where this system is embedded in less sustainable conventional or dominant regimes and landscapes. The need for fundamental changes, in the way that humans interact with nature, is widely argued for in order to achieve sustainable development, and farmers occupy a central role through participation in complex networks of agri-food systems. They have also found themselves disconnected from nature through conventional agri-industrial production practices. Four niches (biological control, ecological restoration, soil health, and ecological pond management) within the greenhouse sector of Almeria (SE Spain) are explored in a case study. Our results indicate that a farmer’s interaction with nature is functional, but through agroecological practices, a deeper understanding of the ecosystems in which greenhouse landscapes are embedded may be gained. As they become more connected to nature and benefit from ecosystem services, they can transition to more sustainable agricultural systems.

Multifunctional agriculture is favoured by EU rural policy and considered as a tool for integrated and sustainable rural development. Recent approaches to define the multifunctional practices of rural farm households (different from those of ‘conventional’ agriculture) according to the use of their resources result in three dimensions: deepening, broadening and regrounding. In this paper, the multifunctional practices of farm households in six rural areas of Greece with different characteristics of agricultural production are examined. Data were obtained from interviews with farmers about the practices of their households. The findings indicate that regrounding practices are encountered more often in all areas and are not associated with the degree of the ‘professionalisation’ of agriculture in each area. Broadening and deepening practices are less common for two reasons: some of these practices take place at a level independent from most Greek farmers, and demand appears to shape some of these practices.