Crucial roles of diluents in semen cryopreservation are to provide a source of energy to sperm, provide electrolytes for osmotic pressure, buffer against extremes in pH and protection from temperaturerelated damage (Omodewu et al., 2021). Even with the addition of the diluent, freezing induces high production of reactive oxygen species (ROS), osmotic stress, damage to spermatic DNA, membrane destabilization and mitochondrial dysfunction (Vichas et al., 2017).Although ROS plays an important role in normal sperm physiology, an imbalance in ROS production and degradation causes adverse effects on spermatozoa (Moichela et al., 2021).Bacterial contamination is another factor that affects semen quality (Gloria et al., 2014). Multiple genera of bacteria have been