The full gene sequence encoding for the Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase (PKA) regulatory (R) subunits was cloned. A poly-His tagged construct was generated [TeqR-like(His)], and the protein was expressed in bacteria and purified to homogeneity. The size of the purified TeqR-like(His) was determined to be ∼57,000 Da by molecular exclusion chromatography indicating that the parasite protein is a monomer. Limited proteolysis with various proteases showed that the T. equiperdum R-like protein possesses a hinge region very susceptible to proteolysis. The recombinant TeqR-like(His) did not bind either [H] cAMP or [H] cGMP up to concentrations of 0.40 and 0.65 μM, respectively, and neither the parasite protein nor its proteolytically generated carboxy-terminal large fragments were capable of binding to a cAMP-Sepharose affinity column. Bioinformatics analyses predicted that the carboxy-terminal region of the trypanosomal R-like protein appears to fold similarly to the analogous region of all known PKA R subunits. However, the protein amino-terminal portion seems to be unrelated and shows homology with proteins that contained Leu-rich repeats, a folding motif that is particularly appropriate for protein-protein interactions. In addition, the three-dimensional structure of the T. equiperdum protein was modeled using the crystal structure of the bovine PKA Rα subunit as template. Molecular docking experiments predicted critical changes in the environment of the two putative nucleotide binding clefts of the parasite protein, and the resulting binding energy differences support the lack of cyclic nucleotide binding in the trypanosomal R-like protein.
Polyclonal immunoglobulin Y (IgY) antibodies were produced in chicken eggs against the purified R(II)-subunit of the cAMP-dependent protein kinase (PKA) from pig heart, which corresponds to the Sus scrofa R(II)α isoform. In order to evaluate whether Trypanosoma equiperdum possessed PKA R-like proteins, parasites from the Venezuelan TeAp-N/D1 strain were examined using the generated anti-R(II) IgY antibodies. Western blot experiments revealed a 57-kDa polypeptide band that was distinctively recognized by these antibodies. Likewise, polyclonal antibodies raised in mice ascites against the recombinant T. equiperdum PKA R-like protein recognized the PKA R(II)-subunit purified from porcine heart and the recombinant human PKA R(I)β-subunit by immunoblotting. However, a partially purified fraction of the parasite PKA R-like protein was not capable of binding cAMP, implying that this protein is not a direct downstream cAMP effector in T. equiperdum. Although the function of the S. scrofa PKA R(II)α and the T. equiperdum PKA R-like protein appear to be different, their cross-reactivity together with results obtained by bioinformatics techniques corroborated the high level of homology exhibited by both proteins. Moreover, its presence in other trypanosomatids suggests an important cellular role of PKA R-like proteins in parasite physiology.
HighlightsSoluble forms of VSGs from seven Venezuelan animal trypanosomes were purified and characterized.All purified soluble VSGs exhibited cross-reactivity with Trypanosoma vivax.Anti-VSG antibodies behaved as markers of infection for non-tsetse transmitted trypanosomes.All purified soluble VSGs can be used as diagnostic reagents for bovine trypanosomosis.
In our search for new safe antiparasitic agents, an enzymatic
pathway
was applied to synthesize a series of N-pyridinylmethyl
amides derived from structurally different carboxylic acids. Thirty
derivatives, including 11 new compounds, were prepared through lipase-catalyzed
acylation in excellent yields. In order to optimize the synthetic
methodology, the impact of different reaction parameters was analyzed.
Some compounds were evaluated as antiproliferative agents against Trypanosoma cruzi, the parasite responsible for American
trypanosomiasis (Chagas’ disease). Some of them showed significant
activity as parasite proliferation inhibitors. Amides derived from
2-aminopicoline and stearic and elaidic acids were as potent as nifurtimox
against the amastigote form of T. cruzi, the clinically
relevant form of the parasite. Even more, a powerful synergism between
nifurtimox and N-(pyridin-2-ylmethyl)stereamide was
observed, almost completely inhibiting the proliferation of the parasite.
Besides, the obtained compounds showed no toxicity in Vero cells,
making them excellent potential candidates as lead drugs.
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