Acalculous cholecystitis has been associated with several infectious agents, but its relation with Plasmodium falciparum infection has not been clearly defined. This is the first case of acalculous cholecystitis produced by Plasmodium falciparum infection that is directly documented and should be included among the differential diagnoses of acalculous cholecystitis.

Basics of isolation and cultivation of chondrocytes according to good laboratory practice ResumenObjetivos: El objetivo del presente estudio era determinar si los condrocitos aislados de cinco pacientes ancianos (edad media 63 años) con artrosis (grado 3) mantienen su proliferación y potencial condrogénico. El aislamiento y cultivo de condrocitos fueron llevados a cabo de acuerdo a los estándares de buenas prácticas de laboratorio. Métodos: Los condrocitos fueron aislados de una biopsia de cartílago mediante digestión enzimática. El cultivo fue llevado a cabo en un ambiente controlado (sala blanca). La caracterización del fenotipo de los condrocitos se logró mediante análisis de citometría de flujo. Resultados: Tras tres semanas de cultivo se podían observar estructuras poligonales propias de los condrocitos, pero también se observaba morfología de tipo fibroblasto en el cultivo. El análisis de la citometría de flujo reveló que el fenotipo de los condrocitos cultivados tras el primer pasaje era positivo para CD44 (98,92%), CD90 (97,11%) y negativo para el marcador hematopoyético CD45 (0,10%). Conclusiones: Los condrocitos articulares humanos obtenidos de cinco pacientes ancianos con artrosis mantenían un fenotipo condrocitario y podrían ser potencialmente utilizados para la implantación autóloga. Las condiciones para el cultivo fueron establecidas de acuerdo a los estándares de buenas prácticas de laboratorio para así minimizar el riesgo de contaminación celular in vitro. AbstractObjectives: The objective of the present study was to determine if chondrocytes isolated from human cartilage of five elderly patients (middle age 63) with osteoarthritis (stage 3) maintain their proliferation and chondrogenic potential. Isolation and cultivation of chondrocytes was performed according to good laboratory practice (GLP) standards. Methods: Chondrocytes were isolated from cartilage biopsy by enzymatic digestion. Cultivation of cells was performed in a controlled environment (cleanroom). Phenotype characterization of chondrocytes was achieved by flow cytometry analysis. Results: Three weeks after cultivation polygonal structures typical for chondrocytes were observed, but spindle/fibroblast like morphology was also detected in culture. Flow cytometric analysis showed that chondrocytes were positive for CD44 (98,35% ± 0,50), CD90 (97,15% ± 0,13) after first passage (P1) and the cells were negative for hematopoietic marker CD45 (0,21% ± 0,11). Conclusions: Human articular chondrocytes obtained from five elderly patients with osteoarthritis maintained a chondrocyte phenotype and could be potentially used for autologous implantation. We have standardized the conditions for cultivation according to GLP standards to minimize the risk of in vitro cell contamination.

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