Los modelos computacionales son un área en continuo desarrollo e investigación que se usan como apoyo a los procedimientos experimentales. Estos representan una herramienta fundamental para definir variables cada vez más específicas a aplicarse reduciendo tiempo de investigación y dinero. En este estudio se desarrolló un modelo computacional 3D de células de fibroblastos y osteoblastos humanas estimulados por campos magnéticos. El modelo se creó a partir del esquema experimental real formado por la fuente (Bobina Helmholtz), una placa FalconTM de 96 pozos y el material celular. La resistencia eléctrica para las células se midió en cada material biológico y en el modelo se asignó la resistividad. La densidad de flujo magnético aplicada fue de 1.0 y 1.5 mT y frecuencias entre 15 y 105 Hz. Las variables evaluadas fueron el campo eléctrico, la densidad de corriente y el calentamiento eléctrico. Se observó un crecimiento exponencial de las señales inducidas con la frecuencia y la densidad de flujo magnético generado, más significativo para el primer caso.
Nowadays and in spite of all the preventive effort made, cardiovascular diseases remain as one of the leading causes of death worldwide. Current treatments decrease the progression of the disease but fail to offer a solution where the contractile function lost due to the injury can be recovered. In response to this, regenerative medicine proposes new approaches and one of this is the use of biomaterials that can mimic the role of the extracellular matrix and provide support for new cells in the affected heart. This study evaluated the repairing effect of collagen type I and MaxGel when injected, alone or combined, into the infarcted heart of Wistar rats. Cardiac function was quantified using the ejection fraction as the main parameter and it was measured in three time points during the study: pre-infarction, post-infarction, and post-treatment. Additionally, histological samples of the hearts were taken for evaluation. The data obtained showed a marked recovery of the cardiac function in the animals were collagen type I was injected with an increase of 8.2% on the mean ejection fraction, suggesting that this biomaterial has the capacity to stop the progressive decline of the cardiac function in an infarcted heart.
Introduction: Ongoing research in the use of electromagnetic stimulation as coadjuvant in fracture healing has led the authors to begin generating computer models in order to predict cellular growth changes when cells are electromagnetically stimulated. By generating these models, scientists will be able to better understand how electromagnetic fields affect cellular development. The experimental design integrated a cellular culture bioreactor along with an external magnetic stimulation system, which allowed for dermal models to be exposed to controlled magnetic fields. Methods: Initially, it was necessary to analyze the static growth of Normal Human Skin Fibroblast (NHSF) cells when they were exposed to Extremely Low Frequency -Electromagnetic Fields (ELF-EMFs). Using optimal conditions for the NHSF culture, from stimulation signal to scaffolding material, we were able to perform the dynamic flow stimulation experiments. Results: The following systems were developed: (1) a bioreactor aimed at cellular tissue culture, and (2) Helmholtz coils capable of generating stimulation signals for the cultured tissue. The authors were able to appreciate the quantified values of cellular density diluted in all the experiment samples that were taken and overall, the irradiated samples displayed an average increase of 53% higher cellular density for the same amount of initial cellular seeding when the cells were exposed to a 1 mT, 60 Hz magnetic field signal. Conclusion: ELF-EMF's indeed alter NHSF cell growth rates and it is the challenge of the authors to continue investigating what cellular mechanisms are altered when cells are exposed to ELF-EMF's.
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