The product mixture labeled A was chromatographed on a silica gel column (200 g) by elution with hexane-diisopropyl ether (4:1). First fractions gave 3b (0.51 g): mp 170 °C (from re-hexanechloroform);

We describe a solid-phase immunochromatographic serologic test, FlexSure® HP, to detect IgG antibodies against Helicobacter pylori. H. pylori colonize the stomach and proximal duodenum, cause ulcer disease and mucosa-associated lymphoid tissue lymphoma, and have a role in the development of other disorders, including gastric adenocarcinoma. FlexSure HP consists of a test strip, conjugate pad, and absorbent pad, in a novel reverse-flow chromatography format. In these studies, FlexSure HP was demonstrated to be specific for IgG antibodies against H. pylori. The reactive cutoff of the test was consistent with [13C]urea breath test and commercially available ELISAs. FlexSure HP had 94% sensitivity, 88% specificity, and 91% accuracy relative to [13C]urea breath test; and 95% sensitivity, 94% specificity, and 95% overall agreement relative to high-molecular-mass cell-associated protein enzyme immunoassay (HM-CAP EIA). FlexSure HP is a simple-to-perform, visually read test requiring no specialized training, equipment, or instrumentation, and yields rapid, accurate, qualitative results.

Aus dem 4‐Hydroxy‐pyrrolidon (I) entsteht das Trifluoracetat (II), das in Gegenwart einer Base in (III) übergeht und dann zu den beiden Pyrroljdonen (IV) und (V) gespalten wird.