Lipid-based delivery systems are widely used to incorporate chemicals with poor solubility. The influence of tocopherol and sesamol on emulsion delivery systems, including their bioactivity, is not well-known. Herein, the overall aim of this study is to understand the effect of tocopherol and sesamol on the physical stability and bioactivity of various emulsions comprised of oil (medium chain triglyceride, coconut oil, palm oil) with different carbon chain lengths using the C. elegans model. All emulsions show good physical stability; droplet size, polydispersity index, zeta potential, and microstructure are relatively constant over the incubation time. Tocopherol and sesamol show the greatest bioactivity (greater lipid-lowering effect and in vivo antioxidant activity) in emulsions fabricated with palm oil. Knowledge on emulsion stability and bioactivity is important for delivery systems in the food industry involving tocopherol, sesamol, and other related bioactive compounds.