The semen movement and sperm head size patterns of boar ejaculates were analysed using computer-assisted semen analysis (CASA)-Mot and -Morph systems. The aim of the present study was to compare morphometric and kinematics variables from boars and to determine the relationship with sow fertility variables related to litter size. The females were from maternal crossing schemes such as the continuous 3-generation cross between York (Y), Landrace (L), and Pietrain (P) hybrid sows and Pietrain boars. Semen samples were collected from 11 sexually mature boars from two sire lines. Samples were analysed using the ISAS ® v1 system to evaluate eight kinematic variables of sperm velocity, progressiveness and undulations. Four morphometric parameters of sperm head size (length, width, area and perimeter) were analysed. Bayesian analysis revealed relevant differences in four kinematic variables (VSL, LIN, STR and WOB) between sire lines, with a probability of relevance (P R ) of 0.79-0.91, and Pietrain boars were associated with higher progressive motility compared with Duroc x Pietrain boars. Moreover, there were relevant differences in all morphometric variables (P R = 0.82-0.85) between sire lines. The dam line Y-L-50 (½ Y × ½ L) had higher total born per litter and piglets born alive, and YLP-75 ( 1 / 8 Y × 1 / 8 L × 3 / 4 P) was associated with higher values of litter weight at birth (highest posterior density region at 95% = 9.92, 16.41 kg). There are relevant differences in kinematic variables between the assessed sire lines and the differences in morphometric and litter size variables were also relevant. The York-Landrace hybrid sows had higher total born per litter and piglets born alive, and there were relevant differences when compared with YLP-50 (¼ York × ¼ Landrace × ½ Pietrain). Differences in kinematic and morphometric variables between sire and dam lines related to fertility need to be further studied.
Ejaculate quality can be regarded as multifactorial, with nutrition being a factor that could directly influence sperm parameters. The present study aimed to evaluate seminal quality associated with seasonal fat-soluble vitamin supplementation of boars. Seven sexually mature boars were randomly allotted to one of the three groups, and fed one of the three supplementary diets for 32 weeks: (1) control treatment (COD), without supplementation of fat-soluble vitamins, (2) treatment containing 100% fat-soluble vitamin supplementation administered intramuscularly, which was based on fat soluble vitamin supplementation (A, D3, E) (FVD1), and (3) treatment containing 50% of fat-soluble vitamin supplementation (FVD12). Semen was collected at 7-day intervals. Semen samples were analyzed to assess several sperm parameters using the Computer-Assisted Semen Analysis (CASA) ISAS®v1 system. Results showed that groups receiving FVD1 and FVD12 supplementation had an increased semen volume. The percentages of motile and progressively motile sperm were increased by FVD1 treatment. A statistically significant interaction between treatment and season was found in the percentage of motility and progressive motility (p < 0.05). Sperm concentrations showed significant differences (p < 0.05) between treatments. Velocity variables (VSL, VCL, and VAP) were higher (p < 0.05) in boars that received fat-soluble vitamin supplementation in comparison to controls receiving no supplementation. The FVD1 treatment presented spermatozoa with greater head size and more elongated heads (p < 0.05). Overall, the utilization of dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates. This highlights the importance of fat-soluble vitamin supplementation in sexually active boars.
La suplementación vitamínica es importante para optimizar el potencial reproductivo del verraco. El objetivo de este estudio fue evaluar la calidad seminal del eyaculado de verracos suplementados con vitaminas liposolubles y el efecto de la durabilidad de las muestras de semen a través del tiempo. Se utilizaron cuatro verracos de una línea comercial terminal (Duroc*Pietrain) con edades promedio de 25,3±8,8 meses y de fertilidad conocida. Las muestras de semen se recogieron por la mañana, una vez por semana. Los animales se asignaron aleatoriamente a dos grupos y se alimentaron con cualquiera de los dos tratamientos durante 16 semanas: (1) tratamiento de control (TC, sin suplementos de vitaminas liposolubles), (2) tratamiento que contenía un 100 % de suplementos de vitaminas liposolubles (TS) a base de vitaminas liposolubles (A, D3, E). Se encontraron diferencias (P<0,05) en la movilidad total del eyaculado para el tratamiento que recibió suplementación vitamínica (TS) (85,11 ± 0,79 %), respecto del tratamiento control (TC) (74,39±0,79 %). Los animales suplementados con vitaminas presentaron mayor movilidad progresiva (P<0,05), con respecto al TC (79,84±0,80 %; 69,63 ± 0,80 %, respectivamente). No hubo diferencias (P>0,05) en los valores de movilidad total y progresiva a las 24 y 48 horas, pero si hubo una disminución significativa (P<0,05) para ambas variables al término de las 96 horas de conservación. Los resultados del TS con respecto al TC en las variables velocidad curvilínea (VCL), velocidad rectilínea (VSL) y porcentaje de linealidad (LIN) fueron mejores (P<0,05), durante 24 horas de conservación. La conservación del eyaculado puede contribuir a la eficiencia del manejo en las granjas porcinas y puede verse afectada por distintos factores, donde la suplementación y la nutrición adecuada, pueden aportar a la calidad seminal del verraco.
El objetivo del estudio fue determinar el efecto de la tasa de fotogramas sobre diferentes profundidades de cámara de recuento espermático en semen de verraco y analizar la distribución de los espermatozoides en los campos longitudinales de la cámara de conteo. Se colectó una muestra de semen a 10 verracos Pietrain de 2-3 años. Se determinaron ocho parámetros de cinética espermática mediante el sistema CASA (Computer-assisted semen analysis)-Mot (ISAS®v1). Se utilizaron cámaras ISAS®D4C con profundidades de 10, 16 y 20 μm a una frecuencia de captura de imagen de 25, 40 y 60 fotogramas por segundo (fps). La profundidad de cámara afectó las variables de cinética espermática, obteniéndose valores más altos a 20 µm. La tasa de fotogramas (FR) presentó una relación positiva con los parámetros de cinética obteniéndose valores más altos a 60 fps, excepto para la amplitud del desplazamiento lateral de la cabeza (ALH) que presentó un comportamiento inverso. La variable más sensible al cambio en FR fue la velocidad curvilínea (VCL), donde el mejor nivel se observó con 80 fps. Se observó una disminución progresiva en los patrones cinemáticos conforme los espermatozoides avanzaban por los campos de las cámaras. Se concluye que el uso de diferentes cámaras de recuento y el incremento en el número total de fps genera cambios significativos en la estimación de parámetros de cinética espermática en el verraco.
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