High copper (Cu) soil contents, due to the continuous vineyard application of Cu fungicides throughout the years, may impair the growth of the shoot and modify the structure of the root system. The current study aimed to investigate the threshold levels of available Cu in the soil causing toxicity effects in young grapevine plants of 'Red Niagara' cultivated in clay soils. Grapevine plantlets were cultivated in pots containing vineyard devoted soils with increasing contents of available Cu (25, 80, 100 and 165 mg kg), for 53 days. Photosynthesis and transpiration rates, and the quantum yield of photosystem II (Fv/Fm) were evaluated during the cultivation period. At the end of the experiment, the plant nutrient and leaf chlorophyll were determined, along with the anatomical analysis of the root system structure and plant dry matter determination. Higher levels of available Cu in the soil increased the apoplastic, symplastic and total fraction of the metal in the roots, reducing the other nutrients, especially in the shoots. Photosynthesis, transpiration rates and Fv/Fm were also reduced. Higher levels of Cu led to anatomical changes in the roots, that increased diameter, number of layers in the cortex, vascular cylinder and total root areas. It also resulted in reduced dry matter production by grapevines.

NUTRITIONAL STATUS, VIGOR AND YIELD OF GRAPEVINES INTERCROPPED WITH COVER CROPSABSTRACT -Perennial or annual species of cover crops can be grown in vineyards to protect the soil surface and to reduce the availability of water and nutrients to the vine. The study aimed to evaluate the nutritional status, vigour and yield of grapevines intercropped with some species of cover crops under two different managements. A field experiment was conducted for two years, 2009 through 2011, in a vineyard of cv Cabernet Sauvignon in a Haplumbrept soil. The following treatments were evaluated: a control, where weeds were controlled by chemical drying in the grapevine row and by mowing in the inter row; a perennial species, tall fescue (Festuca arundinacea); two sequences of annual species, ryegrass-moha (Lolium multiflorum-Setaria italica) and oat-buckwheat (Avena sativa-Fagopyrum esculentum); and two types of crop residues management, with and without its transfer of the grapevine row to the inter row. The grapevine leaves were sampled at flowering and at berries softening stages for analysis of N, P, K, Ca and Mg contents. It was determined the branches and internodes lengths, branches pruned mass, grape bunches length, width and mass, yield per plant and the weight of 100 berries and it was also calculated the Ravaz index of the grapevines. Grapevines intercropped with succession of annual cover crops showed higher total N content in leaves at flowering, vigour and grape yield. The transfer of the crop residue from the row to the inter row, did not affect the grapevine vigour and yield, but decreased the total content of N in the leaves at flowering. The tall fescue as soil cover crop in vineyard can be an effective alternative to reduce the vigor of the grapevine.

Resumo -O objetivo deste trabalho foi avaliar a influência de plantas de cobertura verde sobre a produtividade das videiras e sobre a composição da uva e do vinho. Durante duas safras, foram feitas avaliações de três tipos de consórcio, dois manejos das coberturas e de um tratamento controle, com plantas espontâneas controladas por herbicidas e roçagem. Utilizou-se vinhedo de uvas 'Cabernet Sauvignon', localizado a 1.130 m de altitude, em um Cambissolo Húmico distrófico, em São Joaquim, SC. Os consórcios foram realizados com a sucessão de cultivos anuais de moha (Setaria italica) com azevém (Lolium multiflorum) e de trigo mourisco (Fagopyrum esculentum) com aveia-branca (Avena sativa), bem como com a planta perene festuca (Fetusca sp.). Os manejos consistiram da transferência ou não do resíduo cultural da linha para a entrelinha. As videiras apresentaram maior produtividade de uva no consórcio com as plantas anuais, em comparação ao tratamento controle, ou com a planta perene festuca. O manejo da cobertura verde não teve influência sobre as variáveis avaliadas. Os consórcios não influenciaram de forma consistente os teores de N da uva nem a composição do mosto, embora, na última safra, o teor de sólidos solúveis totais do mosto tenha sido maior nos tratamentos com consórcio, em comparação ao controle. Além disso, as videiras consorciadas com festuca podem proporcionar vinho com maior teor de antocianinas e polifenóis totais.Termos para indexação: Vitis vinifera, adubo verde, qualidade da uva, vigor vegetativo, vinho de altitude. Productivity and composition of grapes and wine of vines intercropped with cover cropsAbstract -The objective of this work was to evaluate the influence of green cover crops on vine productivity and on grape and wine composition. For two growing seasons, evaluations were done for three intercrops, two managements of the cover crops, and for a control treatment with weeds controlled by herbicides and mowing. A vineyard of 'Cabernet Sauvignon', located at 1,130 m altitude in a Haplumbrept soil, in São Joaquim, SC, Brazil was used. Intercropping was done with a succession of the cover crops moha (Setaria italica) with annual ryegrass (Lolium multiflorum), and buckwheat (Fagopyrum esculentum) with oat (Avena sativa), and with perennial fescue (Fetusca sp.). Cover crop managements consisted of transferring or not crop residues from the row to the between rows. Grape yield were higher in vines intercropped with the annual plants, in comparison to the control treatment or to fescue. The management of the cover crops did not affect the evaluated variables. Intercropping did not consistently influence N contents of grapes and wine composition; however, soluble solid contents of must were greater with intercropping treatments compared to control, in the last season. Moreover, vines intercropped with fescue can enable wine with greater content of anthocyanins and total polyphenols.

RESUMO As aplicações sucessivas de dejeto líquido de suínos podem aumentar os teores de cobre e fósforo na