Objective: The objective of this research was to determine the antioxidant and hepatoprotective activity of Abelmoschus manihot L. Medik (AMLM) leaves in carbon tetrachloride-induced liver damage in rats (Rattus norvegicus). Methods: Samples of dried leaf were macerated using 96% ethanol solvent to obtain a viscous extract. Fractionation was then performed using ethyl acetate and n-butanol solvent. The antioxidant activity test of each fraction was done by 1,1-diphenyl-2-picrylhydrazyl assay. For the hepatoprotective activity test, the fractions were suspended in Tween 20 0.4% solution with concentrations of 10 mg/mL and 20 mg/mL. Rats were divided into 6 treatment groups consisting of five rats/group. Group I (positive control) was given an oral suspension of Curcuma® tablet, Group II (negative control) was given Tween 20 0.4% solution, and Groups III and IV (ethyl acetate fraction) and V and VI (n-butanol fraction) were given fraction suspension. On the 1st–7th days, all groups were given the solution treatment, and on the 8th day, all groups were injected with CCl4 intraperitoneally and the treatment was continued until the 11th day. On the 12th day, the blood of rats was taken and then measured the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). Results: The results of the antioxidant test showed that ethyl acetate and n-butanol fraction, respectively, had IC50 of 89.99 and 114.56 ppm.Measurement of SGOT showed the result for Groups I–VI, respectively, of 160±63.62, 260.53±18.98, 154.16±52.78, 177.43±13.70, 120.07±34.80, and 105.23±40.49 IU/L. Measurement of SGPT showed the result for Groups I–VI, respectively, of 101.87±29.24, 108.1±9.04, 57.73±49.05, 106.07±26.45, 66.9±20.05, and 146.63±84.89 UI/L. Conclusion: The results of this research indicated that the ethyl acetate and n-butanol fraction of AMLM leaves have the antioxidant andhepatoprotective activity.

A BSTRACT The egg white was used to induce rat paw inflammation, with inadequate references to explain its mechanism. It's contained protein was identified as an allergen was suspected to trigger an inflammatory reaction. This research was aimed to evaluate the use of egg white as an inflammatory inductor in inflammation animal models through edema profile and histological change. Male Wistar rats were divided into three groups, which were given λ-carrageenan, fresh takes of the hen's egg white, and sterile saline solution. Edema was induced by subcutaneous injection of 0.1 ml of λ-carrageenan (1%), egg white, and sterile saline solution as the control in the hind paw of rats. Paw volume was measured before and then at 1, 2, 3, 4, 5, 6, and 24 h after the inductor injection. Paw tissue was taken for evaluation of rats’ paw histological change. The data were analyzed by one-way ANOVA followed by LSD test. The results of the study showed that the egg white could induce rat paw inflammation. Edema formation began in the 1 st h and reached the peaks in the 2 nd h after the subcutaneous injection of egg white. A number of leukocyte cells were also found in the inflamed paw tissues. Egg white was potential as an edema inductor for animal models of inflammation for the evaluation of new drugs or natural product with anti-inflammation activity.