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Since its first identification in Scotland, over 1000 cases of unexplained pediatric hepatitis in children have been reported worldwide, including 278 cases in the UK 1 . Here we report investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator subjects, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in liver, blood, plasma or stool from 27/28 cases. We found low levels of Adenovirus (HAdV) and Human Herpesvirus 6B (HHV-6B), in 23/31 and 16/23 respectively of the cases tested. In contrast, AAV2 was infrequently detected at low titre in blood or liver from control children with HAdV, even when profoundly immunosuppressed.AAV2, HAdV and HHV-6 phylogeny excluded emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T-cells and B-lineage cells.Proteomic comparison of liver tissue from cases and healthy controls, identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins.HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and in severe cases HHV-6B, may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children.

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A field experiment was conducted during Kharif season 2014 at the Research farm of Soil Science, Allahabad School of Agriculture, laid out in randomized block design, consisted nine treatments and three replications, it was observed that growth and yield of green gram in treatment N 20 P 40 K 40 + FYM @10 t ha -1 and Rhizobium was maximum. Maximum plant height 50.66 cm, number of leaves plant -1 33.00, number of branches plant -1 4.66 at 60 DAS, number of cluster plant -1 9.33, number of pods plant -1 37.33 and total seed yield 12.10 q ha -1 were found to be significant over all other treatment. Adequate plant nutrient supply holds the key for improving the growth and food grain production of crop.

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