Objective: To assess the knowledge, attitude and Practices among healthcare workers towards covid 19 preventive measures at Women and New-born Hospital of the University Teaching Hospitals in Lusaka. Methods: Cross-sectional study conducted at WNH-UTH, Lusaka. Convenient sample of 264 frontline healthcare workers responded to self-administered questionnaire to determine their knowledge, attitudes and practices on COVID 19 preventive measures. Results: Majority (31.9%) of the respondents were aged 25 : 29 years. The study revealed that 63.4% had a good knowledge, 60.3% had positive attitudes and 59.9% had a good practice. Attitude was positively related with practice (r = 0.524, p < 0.001) and knowledge (r = 0.469; p < 0.001). Further, knowledge was positively correlated with practice (r = 0.51; p < 0.001). Bivariate analysis results showed that only high knowledge score (75.6%; p < 0.001) and high attitude score (77.6%; p < 0.001) was associated with an increase in good practice among healthcare workers towards Covid 19 preventive measures. Conclusion: The study showed the need for continued assessment of Knowledge Attitude and Practice among healthcare workers towards Covid 19 preventive measures. It further showed the need of designing interventions aimed at encouraging sustained compliance to preventive measures among healthcare workers to prevent COVID 19 transmission.