Floodplains are among the most precious and threatened ecosystems in the world. The study deals with floodplain soil contamination caused by 8 heavy metals (HMs) (Cd, Co, Cr, Cu, Mo, Ni, Pb, Zn) originating and transported from old mine works along the Štiavnica River in Slovakia. We determined the total HMs content and the HM fractions using BCR sequential extraction method. We selected 12 alluvial sites (AS), two contaminated sites (CS), and one reference site (RS). The sampling points were located within the riparian zones (RZ), arable lands (AL), and grasslands (GL). We confirmed soil contamination by HMs and the related ecological risk by different factors. The contamination by HMs at many AS localities was similar or even higher than at CS localities. The highest contamination factor was calculated for Cu (39.8), followed by Pb (27.4), Zn (18.2), and Cd (7.2). The HMs partitioning in the different fractions at the CS and AS localities revealed that Cd, Zn, and Pb were mainly associated with the exchangeable and reducible fractions, while Cu was mainly associated with the oxidisable fraction. The soil properties were selectively correlated with the HM fractions. Based on the ANOVA results, the effect of different ecosystem types on HM fractions was revealed.

The expanding demand for new critical raw materials can lead to their increased release to the environment in the form of emerging environmental contaminants (EECs). However, there has never been a comprehensive study that takes into account the total EEC content, the content of various EEC fractions, their behaviour in floodplain soils, and potential ecological and human health risks. The occurrence, fractions, and influencing factors of the seven EECs (Li, Be, Sr, Ba, V, B, Se) originating from historical mining in floodplain soils of various ecosystems (arable lands, grasslands, riparian zones, contaminated sites) were investigated. Based on the evaluation of the overall levels of EECs (potentially toxic elements) in comparison to the soil guideline values set by European legislation for Be, Ba, V, B, and Se, it was found that only Be did not exceed the recommended limits. Among the elements analyzed, Li had the highest average contamination factor (CF) of 5.8, followed by Ba with 1.5 and B with 1.4. Particularly concerning was the discovery of a potential serious health risk associated with Li exposure for children, as indicated by hazard quotients ranging from 0.128 to 1.478. With the exception of Be and Se, the partitioning of the EECs into the different fractions revealed that the EECs are primarily bound with the residual fraction. Be (13.8%) had the highest percentage of exchangeable fraction as the most bioavailable in the first soil layer, followed by Sr (10.9%), Se (10.2%), Ba (10.0%), and B (2.9%). The most frequently observed correlations were between EEC fractions and pH/KCl, followed by soil organic carbon and manganese hydrous oxides. Variance analyses confirmed the impact of different ecosystems on EEC total content and fractions.

Arthropods are part of important functional groups in soil and little is known about their composition in differently used agroecosystems across larger spatial scales. We analysed the qualitative and quantitative structure of soil arthropods with emphasis on ground beetles in different agroecosystems in relation to the various factors (soil physical properties) that Slovakia as a highly diversified country offers. Research was conducted in 4 different soil types (Arenosol, Cambisol, Fluvisol, Leptosol) at 6 study sites located in three different ecological zones with two different land use types (arable land – AL and permanent grasslands – PG). Ten orders of soil arthropods were identified, of which the most abundant was the order Coleoptera, specifically the Poecilus cupreus species in the Carabidae family. The analysis of variance confirmed significant effect of land use type on arthropod number and ecological gradient on arthropod biomass and soil temperature. The number and biomass of arthropods was nearly twice as high in PG plots compared to AL plots with the exception of two study sites located in sub-mountain and mountain regions with the dominance and proximity of extensive forest and grassland ecosystems. From the ecological gradient point of view, the highest arthropod biomass was recorded in the mountain ecological zone.

The aim of the study was to analyse the impact of land use and altitudinal gradient including geological conditions on selected soil physical properties with subsequent effect on earthworms as important soil organisms. The research was conducted at three study sites (Očová – OC, Tajov – TA, Liptovská Teplička – LT) situated in the different climatic and natural conditions of Slovakia each with 3 plots differing in land use (arable land - AL, permanent grasslands – PG, forest land – FL). During 2014 over two periods, we measured soil penetration resistance (PR) with total depth of the measurement (DP) and soil moisture (SM). Earthworms were hand sorted counted and weighed. We found out high variability of measured parameters conditioned by time, space (altitudinal gradient) and land use. PR values of all measurements ranged from 0.19 to 5.00 MPa, DP values from 0.02 to 0.80 m and soil moisture from 2 to 50%. Paired samples test confirmed differences between different land use types mainly between AL and FL plots. There were confirmed significant differences between three ecological gradients in all observed properties with one exception. Correlations among observed variables under different altitudinal gradients and land use types were found. The earthworm density and biomass was significantly higher in permanent grasslands compared to forest and arable land. In arable land, the earthworm density and biomass negatively correlated with the penetration resistance and positively with the depth of the total measurements. In permanent grasslands earthworm biomass positively correlated with soil moisture.