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Summary — Vegetative propagation of 34-to 67-year-old Quercus acutissima trees was successfully achieved from rooted cuttings. Semi-hardwood ramets which were obtained from the clones established through grafting twice onto the 2-year-old rootstocks showed 60% rooting (ranging from 20% to 100%). After grafting only once, average rooting frequency was 11% (ranging from 0 to 34%) using cuttings dipped in 500 ppm indole-butyric acid (IBA) solution. The rooting medium consisted of equal volumes of peatmoss and perlite. Cuttings were watered by intermittent mist and grown in the greenhouse at 25 ± 3 °C for more than 5 weeks. For rooting, there was no significant difference between ortet age but marked differences were observed among the clones. Although most of the rooted cuttings did not sprout new buds in the current year, they usually produced slowly-growing buds and/or revealed plagiotropic growth at the following year.