ResumenLos cladóceros, en especial el género Moina, son organismos de gran utilidad para la acuicultura por su valor nutricional y por ser presa fácil para larvas de peces y crustáceos. Sin embargo, su eficiencia reproductiva y velocidad de crecimiento requieren condiciones ambientales específicas, como la intensidad lumínica, la cual es necesaria conocer para definir protocolos de su cultivo, bajo condiciones controladas. El objetivo de este trabajo fue evaluar el efecto de diferentes intensidades de luz, así: T1 = 955 lm; T2 = 750 lm y T3 = 0.0 lm (ambiente totalmente oscuro), sobre algunas variables reproductivas y de crecimiento de la especie, bajo condiciones de laboratorio. Se seleccionaron ejemplares recién eclosionados, los cuales fueron asignados aleatoriamente en los diferentes tratamientos (n= 16). Se alimentaron con una suspensión de microalgas, Scenedesmus sp. (60x10 4 células.mL -1 ), durante 15 días para determinar: periodo de infertilidad Juvenil (IJ), periodo de incubación (PI), fecundidad (F), así como variables morfométricas. El menor tiempo de IJ se observó en el T2 (18.43 ± 2.30 h), siendo diferente cuando fue comparado con el T3 (P<0.05). No se observaron diferencias entre los tratamientos (P>0.05) en las demás variables evaluadas. En conclusión, el cladócero Moina sp constituye una alternativa como alimento vivo por su fácil adaptación a las condiciones de laboratorio, siendo más favorable cultivarlo bajo una intensidad lumínica de 750 lm.Palabras clave: Alimento vivo, lumens, Moina sp., reproducción. AbstractThe cladocerans, Moina gender especially, are very useful organisms for aquaculture because of their nutritional value and because they are easy prey for larval fish and crustaceans; however, reproductive efficiency and growth rate require specific environmental conditions such as certain light intensity, which is necessary to know in order to design appropriate protocols for cultivation under controlled conditions. Consequently, the aim of this ARTÍCULO ORIGINAL/ORIGINAL ARTICLE
Cystatin proteins are known to form a superfamily of cysteine protease inhibitors, which play a key role in protein degradation and are related to different physiological processes, such as development and immunity. Currently, numerous immunoregulatory proteins, such as cystatins, are being used in the control and prevention of diseases in aquaculture. Thus, the objective of this study was to produce recombinant cystatin (rCYST-B) from the red piranha Pygocentrus nattereri and to evaluate its effect on bacterial growth. The gene that encodes cystatin-B was isolated from the spleen of P. nattereri and cloned in an expression system. The protein was produced via a heterologous system involving the yeast Pichia pastoris X-33. The inhibitory activity of purified cystatin-B was evaluated on papain using different concentrations (0–80.0 μg/μL) of rCYST-B. The bacteriostatic action of the protein was evaluated using the Kirby-Bauer method on the growth of Escherichia coli and Bacillus subtilis. rCYST-B showed 100% inhibition at a concentration of 60 μg/μL. Moreover, the bacteriostatic activity of E. coli and B. subtilis showed inhibition of 40.36 and 49.08% compared to the negative control (phosphate buffer), respectively. These results suggest that recombinant CYST-B has biotechnological potential for use in aquaculture.
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