The native Chilean shrubs Buddleja globosa (Matico) and Ribes magellanicum (Zarzaparrilla) are used widely at a rural level, due to their medicinal properties. Nevertheless, little is known about their secondary metabolites and cytotoxic effect. The aim of this study was to evaluate the content of different compounds like catechin, epicatechin, p-coumaric acid and the antioxidant capacity by ABTS, ORAC, FRAP and DPPH methods. In addition, the cytotoxic activity of both extracts was evaluated against Chinese hamster ovary (CHO-K1) cell lines by MTT and neutral red assays. The results suggest that the most abundant constituent in Budleja globosa and Ribes magellanicum were catechin (682.43 mg/100 g DW) and epicatechin (3362.08 mg/100 g DW) respectively; while the ORAC methodology showed an elevated antioxidant activity for Matico (134147.31 μmol Trolox Eq/100 g DW). On the other hand, both extracts at the assayed concentrations affect the membrane stability and cellular metabolic capacity of the CHO-K1 cell lines. These finding provide a direction for further researches, and suggest the Matico and Zarzaparrilla flower extracts as promising sources of antioxidants, and as research objects through the analyze of their metabolic behavior and antitumoral potential.
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El problema más limitante del cultivo de la papa en muchas regiones del mundo, lo constituye la gota o tizón tardío ocasionado por el hongo Phytophthora infestans. Esta investigación se realizó con el objetivo de determinar variaciones poblacionales de este patógeno, basadas en el tipo de apareamiento y en la presencia de razas fisiológicas en 19 aislamientos de P. infestans obtenidos en plantas de papa (Solanum tuberosum) variedades Diacol Capiro, ICA Puracé e ICA Cumanday; papa criolla (Solanum phureja), pepino (Solanum muricatum) y tomate (Lycopersicon esculentum) en diferentes zonas del Departamento de Antioquia (Colombia).Los resultados obtenidos a partir de un apareamiento patrón A1, con los aislamientos colectados en las diferentes zonas sugieren la existencia de una población de P. infestans con un solo tipo de apareamiento. La prueba de virulencia del hongo, realizada en folíolos de 11 diferenciales de papa mostró la presencia de 6 razas fisiológicas, de las cuales la más frecuente fue la 7446 (58%), con los factores de virulencia 1-2-3-4-7-10-11. Aislamientos monozoospóricos presentaron los mismos factores de virulencia que sus respectivos aislamientos recolectados en el campo, lo que sugiere homogeneidad de la población fungosa. La gran complejidad de las razas fisiológicas de P. infestans podría estar influenciada por diversos factores genéticos y medioambientales.Aceptado para publicación: mayo 1997.
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