BackgroundBone marrow mesenchymal stem cells (BM-MSCs) have been identified to be closely associated with tumor growth and progression. However, the roles of tumor-resident MSCs in cancer have not been thoroughly clarified. This study was to investigate the regulating effect of gastric cancer-derived MSCs (GC-MSCs) on gastric cancer and elucidate the underlying mechanism.MethodsGC-MSCs were isolated from primary human gastric cancer tissues and characterized. The effect of GC-MSCs on gastric cancer cell proliferation was analyzed by MTT assay and colony formation assay. Transwell migration assay was performed to evaluate the influence of GC-MSCs in gastric cancer cell migration. The regulating effects of interactions between gastric cancer cells and GC-MSCs on their pro-angiogenic abilities were analyzed in a co-culture system, with the expression, and secretion of pro-angiogenic factors detected by RT-PCR and Luminex assay. Tube formation assay was used to further validate the angiogenic capability of gastric cancer cells or GC-MSCs. Cytokine profiles in the supernatant of GC-MSCs were screened by Luminex assay and neutralizing antibody was used to identify the key effective cytokines. The activations of Akt and Erk1/2 in gastric caner cells were detected by Western blot.ResultsGC-MSC treatment enhanced the proliferation and migration of BGC-823 and MKN-28 cells, which was more potently than MSCs from adjacent non-cancerous tissues (GCN-MSCs) or bone marrow (BM-MSCs). Higher expression levels of pro-angiogenic factors were detected in GC-MSCs than GCN-MSCs or BM-MSCs. After 10 % GC-MSC-CM treatment, BGC-823, and MKN-28 cells expressed increased levels of pro-angiogenic factors and facilitated tube formation more potently than cancer cells alone. Furthermore, GC-MSCs produced an extremely higher level of interleukin-8 (IL-8) than GCN-MSCs or BM-MSCs. Blockade of IL-8 by neutralizing antibody significantly attenuated the tumor-promoting effect of GC-MSCs. In addition, 10 % CM of IL-8-secreted GC-MSCs induced the activations of Akt or Erk1/2 pathway in BGC-823 and MKN-28 cells.ConclusionTumor-resident GC-MSCs promote gastric cancer growth and progression more efficiently than GCN-MSCs or BM-MSCs through a considerable secretion of IL-8, which could be a possible target for gastric cancer therapy.

Four known hydroxyanthraquinones (1-4) together with four new derivatives having a tetralone moiety, namely coniothyrinones A-D (5-8), were isolated from the culture of Coniothyrium sp., an endophytic fungus isolated from Salsola oppostifolia from Gomera in the Canary Islands. The structures of the new compounds were elucidated by detailed spectroscopic analysis and comparison with reported data. The absolute configurations of coniothyrinones A (5), B (6), and D (8) were determined by TDDFT calculations of CD spectra, allowing the determination of the absolute configuration of coniothyrinone C (7) as well. Coniothyrinones A (5), B (6), and D (8) could be used as ECD reference compounds in the determination of absolute configuration for related tetralone derivatives. This is the first report of anthraquinones and derivatives from an isolate of the genus Coniothyrium sp. These compounds showed inhibitory effects against the fungus Microbotryum violaceum, the alga Chlorella fusca, and the bacteria Escherichia coli and Bacillus megaterium.

The threshold method is commonly used to determine cloud in a sky image. This paper evaluates the method by numerical simulation and shows that the aerosol optical depth (AOD) is a key factor that influences the accuracy. Particularly when the visibility is low, a single threshold method is inappropriate. To improve the accuracy of cloud determination from low-visibility sky images, an integrated cloud-determination algorithm is presented that is based on the fast Fourier transform, symmetrical image features, and threshold methods. The preliminary comparison tests show that the new integrated method improves the ability to determine cloud under lower-visibility conditions.

High-resolution and accurate rainfall monitoring is of great importance to many applications, including meteorology, hydrology, and flood monitoring. In recent years, microwave backhaul links from wireless communication networks have been suggested for rainfall monitoring purposes, complementing the existing monitoring systems. With the advances in microwave technology, new microwave backhaul solutions have been proposed and applied for 5G networks. Examples of the latest microwave technology include E-band (71–76 and 81–86 GHz) links, multi-band boosters, and line-of-sight multiple-input multiple-output (LOS-MIMO) backhaul links. They all rely on millimeter-wave (mmWave) technology, which is the fastest small-cell backhaul solution. In this paper, we will study the rain attenuation characteristics of these new microwave backhaul techniques at different mmWave frequencies and link lengths. We will also study the potential of using these new microwave solutions for rainfall monitoring. Preliminary results indicate that all the test mmWave links can be very effective for estimating the path-averaged rain rates. The correlation between the mmWave link measurement-derived rain rate and the local rain gauge is in the range of 0.8 to 0.9, showing a great potential to use these links for precipitation and flood monitoring in urban areas.