The Roman bridge of Alcántara is the largest in Spain. Its preservation is of the utmost importance and to this end different aspects must be studied. The most prominent is the assessment of its structure, and this is especially important as the bridge remains in use. This paper documents the way the assessment of structural safety was carried out. The assessment methodology of existing structures was applied. The preliminary assessment was based on bibliographic data and non-destructive techniques. The geometric data of the bridge were obtained by Terrestrial Laser Scanning (TLS), which made possible the analysis of its deformations and assessment of its structure. Ground-Penetrating Radar (GPR) was also used with different antennae to work at different depths and spatial resolutions with the aim of analysing structural elements. From the above information, the assessment of structural safety was made using the limit analysis method by applying the historical works carried out on it and those described in the regulation of obligatory compliance in Spain (IAP11), studying the sensitivity of safety to the most relevant parameters. The state of preservation and structural integrity of the bridge is discussed and conclusions are drawn on the areas of greatest risk and the bases for the following assessment phase of preservation of the bridge.