The present study examined the efficacy of λ-cyhalothrin, pyriproxyfen and granular formulations of spinosad and temephos for the control of mosquito larvae present in experimental tires in Veracruz State, Mexico in the period 2015–2016. Both λ-cyhalothrin and spinosad granules provided control of larvae and pupae of Aedes aegypti, Ae. albopictus and Culex spp. in used tires in Veracruz State, Mexico, over a 9–12 week period, although numbers of Culex were low. The numbers of Aedes larvae + pupae in pyriproxyfen and temephos-treated tires were slightly less than half of the untreated control tires, probably a result the pupicidal characteristics of pyriproxyfen and possible resistance in the case of temephos. Spinosad was less harmful to predatory Toxorhynchites spp. than λ-cyhalothrin or temephos. The reduced susceptibility to temephos in Aedes populations was confirmed at five other sites in Veracruz. Public health authorities should consider incorporating spinosad as a larvicide in coastal areas at a high risk of dengue, chikungunya and Zika outbreaks in this region.