An acute problem in México and everywhere is the reutilization of sewage polluted by hydrocarbon, such as waste motor oil (O), a toxic waste according to the General Law of Ecological Balance and Environmental Protection and NOM-001- SEMARNAT-1996, NOM-002-ECOL-1997 and NOM-003-ECOL-1997, indicate that the maximum permissible limits of 25 ppm of hydrocarbons in sewage 75 ppm of wastewater to systems of urban sewage and 15 ppm for treated wastewater for public reuse, respectively, which, when exceeding the total of these values, inhibiting the treatment of that domestic sewage. An alternative solution is biostimulation with detergent, minerals and O2 (oxygen) that induce the aerobic heterotrophic microbial population in the sewage to eliminate WMO and reuse it. The objective of this work was the biostimulation of domestic sewage contaminated by AWO until it decreased to a value lower than the maximum of the NOM-001-SEMARNAT-1996, NOM-002-ECOL-1997 and NOM-003-ECOL-1997. For this, the sewage impacted by WMO was diluted and biostimulated with the detergent Tween 80, a mineral solution (MS) and H2O2 as a source of O2, using the response variables: i) CO2 production due to the mineralization of AWO in sewage, ii) determination of the decrease in the concentration of WMO in sewage by gas chromatography coupled to mass (GC-MS) and by Soxhlet, the experimental data was analyzed by ANOVA/Tukey HSD (P ≤ 0.05). The results indicate that the BIS of the water impacted by WMO with Tween 80, MS and H2O2, reduced the concentration to a value of 10 ppm, lower than that established by the NOM-001-SEMARNAT-1996, the NOM-002-ECOL -1997 and NOM-003-ECOL-1997, due to mineralization of the WMO and the evidence of its disappearance according to the CG-EM analysis. This demonstrated the biorecovery of water contaminated by WMO allow industrial and/or recreational reuse

Triticum aestivum requires nitrogen fertilizer (NF) as NH4NO3 for healthy growth, however its excessive application causes loss of soil fertility. An alternative to decrease the amount of NH4NO3 and to optimize is to inoculate T. aestivum seeds with Xanthobacter autotrophicus or/and Bacillus thuringiensis well known asendophytic plant growth promoting bacteria (EPGPB). Both genera and species of EPGPB are able transform metabolic compounds from seeds and roots into phyto hormones, which can be optimized by crude extract carbon nano particles (CECNPs) to enhance the growth of radical system and to improve uptake at 50% dose of NH4NO3 Response variables were: germination percentage, phenology, and biomass to seedling stage. All experimental data were validated by ANOVA/Tukey HSD P<0.05. Seeds of T. aestivum were inoculated with X. autotrophicus and/or B. thuringiensis, plus CECNPs at 50% NH4NO3. Results showed a positive response of T. aestivum seeds to X. autotrophicus and/or B. thuringiensis, with 10 ppm CECNPs, and 50% NH4NO3 enhanced germination percent of 93% in comparison with 73% of T. aestivum when its seeds were not inoculated with these EPGPB fed only with 100% dose of NH4NO3 (relative control); the same positive response of T. aestivum to X. autotrophicus and B. thuringiensis improved by CECNPs at seeding stage compared to T. aestivum used as a relative control.

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Introduction: The growing of maize (zea mays L.) in Latin America is carried out under the intensive production system, which causes loss of soil fertility due to excessive nitrogen fertilizer. Objective: Analyzing the response of maize var. Jala to genera of plant growth promoting bacteria (PGPB) endophytes of maize var. Mexicana (teocintle) when using nitrogen fertilizer such as urea at 50 % under greenhouse and soil conditions. Methodology: A randomized block design was established with five treatments, three controls and twenty repetitions. The treatments with inoculated maize seeds and 50 % urea were: Burkholderia (KO) and Burkholderia sp. 14 (B14); the controls were: non-inoculated maize with 50 % urea (RC1), non-inoculated maize irrigated with water (FC) and non-inoculated maize with 100 % urea (RC2). The experiments were established under greenhouse and soil conditions. The response variables were radical dry weight (RDW) and fresh weight of grain (FWG). ANOVA and Tukey’s mean comparison (P ≤ 0.01) were carried out. Results: In both environments, treatment B14 showed statistically higher values than those obtained with FC (full control), but statistically equal to RC2 (relative control). Study limitations: Maize varieties have specific and beneficial endophytic PGPB genera, which are not necessarily for regional varieties. Originality: PGPB endophytes from teocintle (ancestor of maize) were used because of their close genetic relationship with the maize var. Jala. Conclusion: PGPB endophytes from teocintle are a viable option for sustainable maize production.