The oil of debittered and dehulled grains from two Andean lupins (Lupinus mutabilis) from Peru, Andenes and Común,was extracted by expeller press. The extraction yield and the proximal composition of both varieties were assessed. The oil and the cake, as well as the defatted flour from the variety with the highest extraction yield, werecharacterised for physico-chemical properties, total polyphenol content and antioxidant capacity. The oil shelf-life at 25 °C was extrapolated by Rancimat test. Andenes presented an oil yield of 22.1% (vs. 18.4% of Común),CIELAB colour coordinates of L* = 36.5, a* = 2.2, b* = 20, refractive index and density at 25ºC of 1.469 and 0.903 g/mL,acid number of 3.2 mg KOH/g, free fatty acids content of 1.6%, peroxide number of 2.7 meqO2/kg, p-anisidine number of 1.3, unsaponificable content of 58%, total antioxidant capacity 21.4 μmol TE/g and total polyphenol content of 7.0 mg AGE/100 g. The predominant fatty acid, tocopherol and phytosterol in the oil were oleic acid (56.2%), γ-tocopherol (555 mg/kg) and β-sitosterol (41900 mg/100 g), respectively. The oil shelf-life at 25 ºC was 2.7 years. The Lupinus mutabilis oil extracted by expeller presented an acceptable quality with a lower environmental negative impact than the oils obtained by solvent extraction.

Background. The characterization and evaluation of the nutritional composition of lupine (Lupinus mutabilis) seeds has a long history. However, the determination of the fatty acid profile has only been carried out in a few varieties. Objective. This study determined the fatty acid profile and chemical composition of the oil of six varieties of lupine consumed in Peru. Methods. The extraction of oil from each lupine variety was carried out using the Soxhlet method. Fatty acids, moisture, acidity index, free fatty acids, peroxide value, iodine value, saponification value, and density were determined using official AOAC methods. Results. In general, the average values of saturated fatty acids are lower than those of unsaturated fatty acids. Among the monounsaturated fatty acids (MUFA), oleic acid (C18:1) was the most representative, with values ranging from 41.83% to 54.33%. The “Andenes” variety showed an average oleic acid value of 54.60% compared to the other varieties. The composition of polyunsaturated fatty acids (PUFA) was dominated by linoleic acid (C18:2), and the variety “Cholo fuerte” reported higher values (34.70%) compared to the other varieties. Likewise, the highest average PUFA was 36% and was observed in the “Cholo fuerte” variety. The concentration of linolenic fatty acid (C18:3) ranges from 2.1% for the “Andenes” variety to 2.9% for the common lupine. Additionally, the mean content of linolenic (ω-6) and linoleic (ω-3) acids was 2.33 and 30.89% (13 : 1 ratio), respectively, with the consequent ratio of ω-6/ω-3 fatty acids at the mean level of 0.08. All physicochemical characteristics of lupine seed oil are in accordance with the requirements for edible oils. Conclusion. These findings make the six lupine varieties a new source of promising food components of high nutritional value.