The demand for healthy foods has also increased the demand for wild and farmed tambaqui since it is a fish rich in omega-3, -6 and -9. To determine which of the two types of fish has the best nutritional quality and thus test the hypothesis that there are no nutritional differences between the groups of fish evaluated, the method of ultraviolet absorption spectrophotometry was used. For this, tambaqui from different environments (wild and farmed) were obtained in the states of Amazonas and Rondonia, Brazil. The fish groups showed differences in the concentrations of omega-3, -6 and -9 (ANOVA, F (8.30) = 16.213, and p < 0.01), both between states and between environments. The wild fish of the Amazonas state presented the best quality meat, and exhibited the highest concentrations of omega-3 (0.223 g ± 0.05 g) and omega-9 (0.208 g ± 0.04 g), which also implies the presence of omega-6, while the other group of fish exhibited the lowest values of omega in their composition.