Factor V Leiden (FVL) R506Q and Prothrombin G20210A are clinically important genetic mutations associated with increased susceptibility to venous thrombosis. The objective of our study was to design a polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) reaction that allows simultaneous detection of these two mutations. The reaction can be used in routine diagnostic settings. We have analysed 4504 alleles for each mutation with a mutagenic primer-based PCR system with a low failure rate. The system eliminates the false positive FVL G1691A results associated with other PCR/RFLP caused by rare confounding mutations adjacent to restriction endonuclease recognition sites. This multiplex PCR/RFLP reaction is rapid, robust and dependable.

A previously unreported finding of polypropylene mesh erosion into the bladder two years after laparoscopic colposuspension. As with the vagina, synthetic mesh can erode into the bladder following urogynaecological sling procedures. Previous surgery may be a risk factor. Recurrent urinary tract infection or persistent haematuria following such a procedure should raise suspicion of mesh erosion.