No abstract

Seven strains of Mycoplasma hominis from human genital tracts were selected to produce antisera. The complement-dependent mycoplasmacidal (MC) activity and two complement-independent activities, metabolic inhibition (MI) and agglutination during growth (GA), of these sera were compared. The GA and MI tests were equivalent in titer and generally not affected by concentration of antigen; they revealed specific cross-reacting patterns among the seven strains. There was little if any cross-reactivity with antisera to other human mycoplasmas or to Mycoplasma arthritidis. Control sera from unimmunized rabbits and sera against concentrated uninoculated culture media were also nonreactive. On the other hand, the sensitivity and specificity of the MC test were affected by the concentration of antigen: at about 10 2 color-changing units (CCU) of antigen, all strains were equivalently cross-reactive against the seven antisera, but at 10 2 to 10 4 CCU the individual strain specificity appeared, whereas at about 10 5 CCU little or no antibody was detectable by MC. The MC tests with the lowest concentrations of antigen gave the highest titers of antibody, but at such low concentrations of antigen there was not only loss of individual strain specificity but also some cross-reactivity against other human mycoplasmas, M. arthritidis , or control rabbit sera. Studies with paired human sera also indicate that the MC test with small amounts of antigen is more sensitive than GA-MI.

No abstract

Membrane and cytoplasmic fractions of Mycoplasma hominis inhibited the multiplication of this mycoplasma. Arginine deiminase (EC 3.5.3.6), isolated from both fractions, reproduced the inhibition. The purified cytoplasmic deiminase had a subunit Mr of 49,000, a specific activity of 53 units (mg protein)-1 and an A280/A260 ratio of 1.76. The membrane-associated enzyme had an identical Mr but lower values for specific activity [39 units (mg protein)-1] and the A280/A260 ratio (1.46). In experiments in vitro, recent clinical isolates of M. hominis produced less arginine deiminase, but grew faster than the laboratory reference strain PG 21. In addition, other growth inhibitory components associated with membrane preparations were detected in recent clinical isolates but were absent from strain PG 21.