Cellulose dimethylphenylcarbamate (CDMPC)-immobilized zirconia (CDMPCZ) was used as a chiral stationary phase for enantioseparation of a set of nine racemic compounds in reversed-phase CEC. Influences of the type and composition of organic modifier and the applied voltage on enantioseparation were examined. Separation data on CDMPCZ were also compared with those on CDMPC-immobilized silica (CDMPCS). Enantiomers of the analytes investigated are well separated in ACN/phosphate buffer mobile phases. Better enantioselectivity and resolution were obtained with ACN than MeOH as the organic modifier. Retention was longer but better enantioselectivity and resolution were obtained on CDMPCZ than CDMPCS.

(S)-N-(3,5-Dinitrobenzoyl)leucine-N-phenyl-N-propylamide-bonded silica was used as a chiral stationary phase for separation of enantiomers of some racemic pi-acidic and pi-basic amino acid amides in normal phase capillary LC (CLC) and CEC. For generation of EOF in CEC, different amounts of water were added to n-hexane-isopropanol (IPA) eluents. Influences of added water and composition of polar modifier on retention, enantioselectivity and resolution were studied. Generally better separation was obtained in the eluents of lower water content. Shorter retention and better enantioselectivities were observed in the eluents of higher IPA content, whereas resolution became lower as IPA content was increased. Enantioselectivities were comparable but resolutions were much better in CEC than CLC.

(S)-N-(3,5-dinitrobenzoyl)leucine-N-phenyl-N-propylamine-bonded silica was used as a chiral stationary phase for separation of a set of racemic pi-acidic and pi-basic alpha-amino acid amides in electrolyteless ACN-water eluents by CEC in the RP and polar organic (PO) modes. The effect of the amount of water in the ACN-water eluent on chiral separation was examined. As water is added to ACN, retention was shortened but resolution and selectivity deteriorated severely. Retention, enantioselectivity, and resolution factors obtained in 100% ACN were compared with those in an n-hexane-isopropanol eluent with a small amount of water by normal phase (NP) CEC. Much shorter retention times with comparable enantioselectivities were observed with 100% ACN, demonstrating the advantage of separation on (S)-N-(DNB)leucine-N-phenyl-N-propylamine-bonded silica in PO-CEC over NP-CEC.