No abstract

Synechococcus elongatus PCC7942 represents a classical microbial model that has been widely studied. Its genome-scale metabolic network was recently published (iSyf715), allowing the integration of high-throughput data with the network connectivity. The high-dimensionality of this analysis paves the way not only for the designing of metabolic engineering strategies but also for the principles elucidation of certain cellular processes functioning. Here we present a reporter metabolites analysis of transcriptional profile of S. elongatus PCC7942 under inorganic carbon acclimation by the integration with the connectivity structure of iSyf715. The analysis is based on the published transcriptional changes after 6 and 24 hours of cells growth, when the CO 2 concentration was shifted from high to low levels. The Reporter Features algorithm was applied to unveil the 52 scattered reporter metabolites (p<0.05) across the whole cyanobacterium metabolism, which could represent key regulatory nodes during the CO 2 regime transitions. In addition, clusters of metabolic genes that significantly and coordinately change their expression during this perturbation were identified by using the Reporter Subnetwork algorithm. These results could support the subsequent inference of feasible transcriptional regulatory maps as well as the assessment of the metabolic network capabilities of iSyf715.

No abstract

ResumenObjetivo: Este artículo se sustenta teórico y metodológicamente en la necesidad de continuar utilizando las tecnologías de la información para la salida a los contenidos curriculares de la asignatura de Química, con el propósito de preparar a los graduados de la carrera de Agronomía en la educación universitaria para responder a las transformaciones que experimenta el modelo económico cubano, dirigidas a revolucionar la agricultura y llevarla a elevados índices de eficiencia productiva. Metodología: Se empleó el método hipotético-deductivo así como el método histórico-lógico y el dialéctico. Resultados: Se relacionan algunas regularidades que afectan la correcta inclusión de las tecnologías en la concepción didáctica del proceso de enseñanza-aprendizaje de los contenidos de los programas de Química en los diferentes años de la carrera. Se presenta un breve análisis de los elementos esenciales que desde el punto de vista teórico sustentan la mediación pedagógica en el uso de las tecnologías de la información. Conclusión: Las nuevas tecnologías informáticas influyen de forma directa y positiva en el aprendizaje desde la clase de química. Palabras clave: mediación pedagógica, tecnologías de la información. AbstractObjective: This article is theoretically and methodologically based on the need to continue to use information technology to output to the content of the Chemistry course. All with the purpose of preparing graduates agronomy career in higher education to meet the changes that the Cuban economic model, designed to revolutionize agriculture and bring to high levels of productive efficiency. Methodology: It was used the hypothetical-deductive method as well as the historical and logical and dialectical method. Results: Some regularities that affect the correct inclusion of technology in the educational concept of the teaching-learning process of the chemistry´s contents programs in different years of the course are related. A brief analysis of the essential elements from the theoretical viewpoint underlying the pedagogical mediation in the use of information technologies is presented. Conclusion: The new information technologies have a direct and positive effect on learning from chemistry class.