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ABSTRACT:The purpose of this study was to evaluate the production of biogas, as well as the biogas production potential resulting from the anaerobic biodigestion of dairy cattle manure under organic (CMOS) and conventional (CMCS) production system. Also, the concentration of thermotolerant coliforms was evaluated after the biodigestion process. Therefore, bench biodigesters prototypes were supplied with CMOS and CMCS for 30 weeks. The experimental design was completely randomized with four repetitions for each treatment. Analysis of total solids (TS), volatile solids (VS), biogas production potential, most probable number (MPN) of thermotolerant coliforms were made. The cumulative biogas production was 6.18 L and 11.15 L, when using the CMOS and CMCS, respectively. Average biogas production potential of CMCS were 0.2; 2.6 and 2.9 L kg -1 substrate, ST and SV added, respectively and for CMOS 0.1; 1.4 and 1.9 L kg -1 substrate, ST and SV added, respectively. After the anaerobic biodigestion process of CMOS and CMCS, it was observed that the concentrations of thermotolerant coliforms were well below than the limit established by law.

RESUMONeste trabalho a meta principal foi avaliar a suscetibilidade dos adultos de Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) e Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) e a qualidade fisiológica dos grãos de milho submetidos ao tratamento com ozônio, em diferentes camadas da massa de grãos, razão pela qual se construíram gaiolas de 3,0 cm de altura e 15,0 cm de diâmetro, em PVC, sendo o fundo e a tampa confeccionados em tecido do tipo organza; as gaiolas foram dispostas em recipientes cilíndricos, também em PVC, com 20 cm de diâmetro, 100 cm de altura e conexões para injeção e exaustão do gás ozônio. Tanto os insetos quanto os grãos de milho foram submetidos a uma atmosfera modificada com 50 mg kg -1 de ozônio injetado a um fluxo contínuo de 8,0 L min -1 em conexão localizada na base (plenum) do recipiente, em diferentes períodos de exposição. A espécie que se mostrou mais susceptível foi a S. zeamais, independentemente da sua distribuição na massa de grãos. O menor período de exposição para controlar 95% dos adultos de S. zeamais e T. castaneum foi de 23,76 e 64,19 h, respectivamente, quando distribuídos próximos à injeção do gás ozônio; em geral, os tratamentos com atmosfera modificada com 50 mg kg -1 de ozônio não afetaram a qualidade fisiológica dos grãos de milho. Palavras-chave: atmosfera modificada, insetos, fumigação Ozone application against Sitophilus zeamaisand Tribolium castaneum on stored maize ABSTRACTThe main goal of this work was to assess the susceptibility of adults of Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and the physiological quality of maize grains subjected to ozone treatment in different grain layers. PVC cages 3.0 cm high and 15.0 cm diameter were built with their bottom and cover closed with organza-like cloth; the cages were placed in cylindrical containers of PVC with 20 cm diameters and 100 cm heights and connections for injection and exhaustion of the ozone gas. Insects and maize grains were subjected to a modified atmosphere with 50 mg kg -1 ozone injected at a flow of 8.0 L min -1 through a connection at the bottom of the container (plenum) for different exposure times. Sitophilus zeamais was the most susceptible species regardless of its distribution in the grain mass. The exposure periods required to achieve 95% control of adults of S. zeamais and T. castaneum were 23.76 and 64.19 h respectively, when distributed near the ozone injection; overall the treatments with atmosphere modified by ozone injection at 50 mg kg -1 did not affect the physiological quality of maize grain.

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