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Land flatworms usually show low ability to disperse and high endemicity, displaying many singletons in studies on land planarian assemblages. Thus, many species have been described based on specimens sampled in a single locality and/or on a few specimens. Based on phylogenetic analyses of concatenated COI and 18S rRNA genes and morphological analyses, a new genus and species of geoplaninid land planarian is described from central–east Argentina and southern Brazil. Winsoria gen. nov. shows, among its most outstanding features, a ventral cephalic retractor muscle and a subneural muscle layer that extends throughout the anterior region of the body. In addition, characters of the reproductive system and the phylogenetic analyses support the erection of this new genus. According to molecular phylogenies,Winsoria bipatria sp. nov. is closely related to species of Luteostriata, Supramontana and Issoca, taxa that also possess a cephalic retractor muscle. Despite its disjunct distribution, phylogenetic analyses, genetic divergence and morphological features show that the allopatric populations studied herein belong to a single species. We argue that the occurrence of W. bipatria in localities separated by hundreds of kilometres and a geographical barrier should be explained by passive dispersal.

BackgroundGalileo is one of three members of the P superfamily of DNA transposons. It was originally discovered in Drosophila buzzatii, in which three segregating chromosomal inversions were shown to have been generated by ectopic recombination between Galileo copies. Subsequently, Galileo was identified in six of 12 sequenced Drosophila genomes, indicating its widespread distribution within this genus. Galileo is strikingly abundant in Drosophila willistoni, a neotropical species that is highly polymorphic for chromosomal inversions, suggesting a role for this transposon in the evolution of its genome.ResultsWe carried out a detailed characterization of all Galileo copies present in the D. willistoni genome. A total of 191 copies, including 133 with two terminal inverted repeats (TIRs), were classified according to structure in six groups. The TIRs exhibited remarkable variation in their length and structure compared to the most complete copy. Three copies showed extended TIRs due to internal tandem repeats, the insertion of other transposable elements (TEs), or the incorporation of non-TIR sequences into the TIRs. Phylogenetic analyses of the transposase (TPase)-encoding and TIR segments yielded two divergent clades, which we termed Galileo subfamilies V and W. Target-site duplications (TSDs) in D. willistoni Galileo copies were 7- or 8-bp in length, with the consensus sequence GTATTAC. Analysis of the region around the TSDs revealed a target site motif (TSM) with a 15-bp palindrome that may give rise to a stem-loop secondary structure.ConclusionsThere is a remarkable abundance and diversity of Galileo copies in the D. willistoni genome, although no functional copies were found. The TIRs in particular have a dynamic structure and extend in different ways, but their ends (required for transposition) are more conserved than the rest of the element. The D. willistoni genome harbors two Galileo subfamilies (V and W) that diverged ~9 million years ago and may have descended from an ancestral element in the genome. Galileo shows a significant insertion preference for a 15-bp palindromic TSM.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-792) contains supplementary material, which is available to authorized users.