Appropriate hygiene of dentures contributes to the maintenance of oral and systemic health. However, most of denture wearers had never been instructed on how to perform daily oral/denture care. The purpose of this study is to investigate the influence of a guidance material associated or not to the provision of hygiene devices and its effect on the habits of denture wearers. Forty-eight complete denture wearers were randomly divided into three groups. Group N received no guidance on how to clean and wear their dentures, group G received an illustrated leaflet and verbal complementary instructions and group GK received the same guidance as group G and a hygiene kit for denture cleaning. After a six to ten week period they responded to a 'hygiene and wearing habits' questionnaire. Chi-square test was used for statistical analysis considering p < 0.05. Brushing was the most frequent cleaning method, the majority of groups N and G used toothpaste. Participants of group G and GK presented low percentages of individuals with hygiene and wearing routine considered improper. The differences between groups in hygiene and wearing routine were statistically significant (p < 0.001). The proposed guidance material yielded better results when associated to provision of hygiene devices for denture cleaning.