SUMMARYThe timing of cell and nuclear division of certain enteric bacteria was determined under conditions of balanced growth. Organisms were grown in a high refractive index medium and photographed at frequent intervals with a phase-contrast microscope. This allowed an estimation of the time between successive divisions of nuclei and cells (interdivision times) and the growth rate of each individual. The interdivision times of cell and nuclear divisions had a similar degree of variation (coefficients of variation of about 20%). The interdivision times of sister cells and sister nuclei were positively correlated to a significant degree. The correlation between mothers and daughters was negative to a significant degree in some, but not all, experiments. The correlation between interdivision times of a cell and that of its corresponding nucleus was positive in most experiments.The rate of mass increase of individual cells was estimated by measuring the rate of elongation. Within the limitations of the method of observation, it could be concluded that cells grew exponentially between successive divisions. Different individuals grew with very nearly the same rate constant. The variation in size of cells at the time of nuclear and cell division was smaller (coefficients of variation of about 10 yo) than that of the interdivision times.Some observations on the morphological changes of nuclei during growth and division are presented.

A comparison is made of the sensitivities for detecting hydrogen with Pt-coated single ZnO nanorods and thin films of various thicknesses (20–350 nm). The Pt-coated single nanorods show a current response of approximately a factor of 3 larger at room temperature upon exposure to 500ppmH2 in N2 than the thin films of ZnO. The power consumption with both types of sensors can be very small (in the nW range) when using discontinuous coatings of Pt. Once the Pt coating becomes continuous, the current required to operate the sensors increases to the μW range. The optimum ZnO thin film thickness under our conditions was between 40–170 nm, with the hydrogen sensitivity falling off outside this range. The nanorod sensors show a slower recovery in air after hydrogen exposure than the thin films, but exhibit a faster response to hydrogen, consistent with the notion that the former adsorb relatively more hydrogen on their surface. Both ZnO thin and nanorods cannot detect oxygen.

We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.

No abstract