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Understanding the spatial and temporal variation of nutrient concentrations, loads and their distribution from upstream tributaries is important for management of large lakes and reservoirs. The Min River is an important tributary of the upper reaches of the Three Gorges Reservoir (TGR) that contributes about 20 % of the inflow water from the Yangtze River to the TGR. It is also one of the main phosphorous sources of the TGR. In this study, total phosphorous (TP) concentrations and hydrological data from 2004 through 2014 for 13 sites in Min River and its tributaries were analyzed. The result suggested that the concentration of TP was comparatively lower in the upstream of the Min River and Dadu River, but tended to increase in recent years. Additionally, the TP pollution status in the Min River basin has gradually evolved from local pollution to whole-basin pollution. The concentration of TP in the Min River main stream increased, decreased and then increased again along the waterway. Pollution from nonpoint sources was the main contribution in the upstream portion and Outer River basin of Min River. Pollution from point sources was more serious than that from nonpoint sources in Chengdu, Meishan and Leshan segment. Specifically, TP pollution in Min River of Chengdu segment (Huanglongxi, Inner River) was primarily affected by urbanization, while Leshan segment (Yuebo) was mainly affected by glyphosate production. Controlling pollution from nonpoint sources and improving the sewage collection rate to reduce TP loads will be the primary task in the upstream portion and Outer River basin. Point source pollution controlling will still be the primary task associated with water quality management in the Inner River (Chengdu segment) and the downstream (Leshan segment) basin of Min River for the foreseeable future. Pollution from both point sources and nonpoint sources should be cared in the other area of Min River basin.

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This work attempted to identify if microalgal biomass can be utilized as an alternative nutrition source in aquaculture feed by analyzing its nutritional value and the anti-nutritional factors (ANFs). The results showed that Chlorella pyrenoidosa contained high-value nutrients, including essential amino acids and unsaturated fatty acids. The protein content in C. pyrenoidosa reached 52.4%, suggesting that microalgal biomass can be a good protein source for aquatic animals. We also discovered that C. pyrenoidosa contained some ANFs, including saponin, phytic acid, and tannins, which may negatively impact fish productivity. The high-molecular-weight proteins in microalgae may not be effectively digested by aquatic animals. Therefore, based on the findings of this study, proper measures should be taken to pretreat microalgal biomass to improve the nutritional value of a microalgae-based fish diet.

No abstract