Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses.Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.Conclusion: The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a high-sensitivity and high-accuracy disposable electrode and evaluated it for several target viruses. Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-codoped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which wassuccessfully applied for the detection of WSSV over a wide linear range of 1.45 × 10 2 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion: The engineered sensor electrode can retain the detection activity for up to 5 weeks, a vital long-term stability requirement for disposable sensing applications. This is the first demonstration of the detection of WSSV by ananofabricated sensing electrode with high sensitivity, selectivity, and stability, providing a potential diagnostic tool to monitor WSSV in the aquaculture industry.

Background: With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a high-sensitivity and high-accuracy disposable electrode and evaluated it for several target viruses.Results: Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-codoped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of 1.45 × 102 to 1.45 × 10⁵ DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.Conclusion: The engineered sensor electrode can retain the detection activity for up to 5 weeks, a vital long-term stability requirement for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing a potential diagnostic tool to monitor WSSV in the aquaculture industry.