The pluripotency of mouse embryonic stem (ES) cells is maintained by self-renewal. To screen for genes essential for this process, we constructed an RNA interference (RNAi) library by inserting subtracted ES cell cDNA fragments into plasmid containing two opposing cytomegalovirus promoters. ES cells were transfected with individual RNAi plasmids and levels of the pluripotency marker Oct-4 were monitored 48 hours later by real time RT-PCR. Of the first 89 RNAi plasmids characterized, 12 downregulated Oct-4 expression to less than 50% of the normal level and 7 of them upregulated Oct-4 expression to more than 150% of the normal level. To investigate their long-term effect on self-renewal, ES cells were transfected by these 19 RNAi plasmids individually and G418-resistant colonies were subjected to alkaline phosphatase (AP) staining after 7 days selection. Except for 4 plasmids that caused cell death, the ratio of AP positive colonies was repressed to less than 60% of the control group by the other 15 plasmids and even below 20% by 10 plasmids. The cDNA fragments in these 10 plasmids correspond to eight genes, including Zfp42/Rex-1, which was chosen for further functional analysis. RNAi knockdown of Zfp42 induced ES cells differentiate to endoderm and mesoderm lineages, and overexpression of Zfp42 also caused ES cells to lose the capacity of self-renewal. Our results indicate that RNAi screen is a feasible and efficient approach to identify genes involved in ES cells self-renewal. Further functional characterization of these genes will promote our understanding of the complex regulatory networks in ES cells.

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For visible light communication (VLC), the light signals are transmitted without optical fibers or any sort of wave-guiding. Due to the inherent broadcast nature, physical-layer security emerges as a promising method to protect information delivery from eavesdropping. As for the secrecy capacity of VLC channel, there exist two features. In one way, the limited optical power makes the common capacity expressions in radio-frequency (RF) communication unapplicable for VLC. In another way, several correlated geometrical parameters directly alters the Lambertian model of indoor VLC channel, which gives the secrecy capacity more meanings. However, the issue considering both aspects has not been studied recently. In this paper, from the practical scenarios, we extract a typical geometrical model to reveal the mobility principles of the legitimate receiver and the eavesdroppers. Then, we character two typical distributions of the geometrical parameter. Correspondingly, we derive the upper and lower bounds on the average secrecy capacity, which have the closed forms. Finally, simulation results show that our upper and lower bounds are tight at high optical signal-to-noise rates (OSNRs). Moreover, the geometrical features of VLC systems and distribution parameters of the receiver mobility are effectively reveal by the bounds.