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The global spread of COVID-19 has disrupted the normality of people’s daily lives, leading the population to social distancing and isolation. The closure of green areas also affected the well-being of the individual during the COVID-19 pandemic. Viewing flowers is expected to have similar positive effects to viewing natural scenery. Therefore, this study investigates how white, red, and yellow flower colors affect individuals’ psychological and physiological well-being. The experiment was conducted in an office-like setting with 50 participants. Participants looked at each flower color for 3 min. Electroencephalograms (EEGs), heart rate variability, and skin conductivity were measured to evaluate physiological responses along with both the semantic differential questionnaire (SD) and the Profile of Mood States (POMS) to assess psychological responses. EEGs showed that the mean values of alpha relative power in the prefrontal lobe were significantly higher when viewing yellow and red flowers vs. white flowers. Furthermore, heart rate variability revealed that viewing yellow and red flowers increased parasympathetic nerve activity significantly. After viewing the yellow and red flowers, the average results for each subscale of the POMS questionnaire improved. The vigor (V) subscale and overall mood status values were significantly improved. The results of the SD method revealed that viewing yellow and red flowers resulted in a significantly higher sense of relaxation, cheerfulness, and comfort than viewing white flowers.