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A tiling integral floating display system is proposed using the optimization of viewing window. The integral floating display, which is a combination of an integral imaging system and a floating lens, has many advantages, but it is hard to realize a large system due to the cost and difficulties in making the large floating device. In this paper, the stackable integral floating display is proposed by modifying the size of the viewing window to be the same as that of the display device, which can be a solution for the large scale autostereoscopic three-dimensional display system.

We propose Depth Cube Display (DCD) method using depth map. The structure of the proposed method consists of two parts: A projection part composed of projector for generating image and a Twisted Nematic Liquid Crystal display (TN-LCD) as polarization modulating device for adjusting the proper depth and a display part composed of air-spaced stack of selective scattering polarizers which make the incident light to scatter selectively as the polarization of light rays. The image from projector whose depth is determined as passing through the TN-LCD displaying depth map progresses into the stack of selective scattering polarizers and then three-dimensional image is generated. At that time, the polarization of each polarizer is set 0°, 45° and 90° sequentially, and then the incident light rays are scattered by different polarizer as the polarization of these rays. If the light ray has the polarization between those of polarizers, this light ray is scattered by multi polarizers and the image of this ray is generated on gap between polarizers. The proposed method is more simple structure and implemented easily than previous DCD method.

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We previously developed an in vivo prospective assay for identification of non-cultured cells with MSC potential. Using this assay we identified a population of cells that were slowly cycling and of low density that were capable of multilineage differentiation both in vitro and in vivo (Z. Wang et al, Stem Cells. 2006 24(6):1573). Further characterization of these cells suggested that they resemble a homogenous population of rare Lin−/Sca-1+/CD45− cells that have the morphology and express several markers of undifferentiated embryonic-like stem cells. In vitro the Lin−/Sca-1+/CD45− cells may differentiate into cells from all three germ-layers (M. Kucia et al, Leukemia. 2007 21(2):297). To determine the in vivo fate of this population, we transplanted 500 or 5,000 Lin−/Sca-1+/CD45− cells from a GFP mouse into SCID mice in each group (n=3) immediately after cell sorting to evaluate tissue generation in vivo. At 4 weeks the regenerative potential of these populations was evaluated by micro-CT and histology, and cells were tracked by gross examination of the harvested tissues by fluorescent microscopy. The results showed that a large number of GFP+ cells are located in the implants, indicating that the transplanted cells maintain the ability to contribute to the generation of new tissue. Bone-like tissue was observed in the Lin−/Sca-1+/CD45− group with as low as 500-cells/implant, while 5,000 Lin−/Sca-1+/CD45− cells generated significantly larger mineralized tissue volume, which was confirmed by micro-CT. Lin−/Sca-1+/CD45+ cell only implantation did not form any mineralized tissue, however, while mixed with 2x106 whole bone morrow cells, positive mineralized tissue occurred. Whole bone marrow mixture also improve bone formation in Lin−/Sca-1+/CD45− cell implants compared the actual bone volumes measured by micro-CT. This study demonstrates that non-cultured BM-derived Lin−/Sca-1+/CD45− cells exhibit the capacity to form bone in vivo with as low as 500 cells/implant. Whole bone marrow mixtures can enhance the bone formation, presumably through the interaction of other populations cells. Based on these findings, it is proposed that non-cultured BM-derived Lin−/Sca-1+/CD45− cells are enriched osteogenic cells that can be applied to bone regeneration in vivo.